气体等离子体诱导的葡萄糖的氧化转化
Mohsen Ahmadi1, Kai Masur1, Sander Bekeschus1,2
1ZIK Plasmatis, Leibniz Institute for Plasma Science and Technology (INP), Felix Hausdorff-Str. 2, 17489 Greifswald, Germany.
Biomedicines
|November 27, 2025
概括
冷气等离子体处理在相关度下产生各种葡萄糖氧化产物 (GOP). 这些GOP提供了关于生物系统和疾病中的氧化应激的见解.
科学领域:
- 生物化学 生化学
- 化学生物学 化学生物学
- 血医学是一种血医学.
背景情况:
- 葡萄糖是一种关键的代谢碳水化合物,容易发生氧化变化.
- 氧化应激条件,包括炎症和活性氧/物种 (RONS),可导致葡萄糖氧化产物 (GOP) 的形成.
- GOPs具有潜在的生物相关性和毒性,影响细胞功能.
研究的目的:
- 系统地描述在受控氧化条件下形成的葡萄糖氧化产物 (GOP).
- 用气体等离子体技术作为氧化模型研究GOP形成.
- 在生理学和病理学相关的葡萄糖度下分析GOP概况.
主要方法:
- 暴露D-葡萄糖溶液 (0.25毫米,2.5毫米,25毫米) 给大气压的等离子体 (kINPen).
- 使用高分辨率质谱法 (HRMS) 和双重质谱法 (MS/MS) 评估随时间的推移而发生的葡萄糖氧化.
- 根据质量与电荷比率和碎片化模式识别和量化GOP.
主要成果:
- 气体等离子治疗诱导了各种各样的GOP,包括2-keto-D-glucose,3-deoxyglucosone (3DG),3,4-dideoxyglucosone-3-ene (3,4DGE),furaldehyde,methylglyoxal和乙.
- 随着初始葡萄糖度和血治疗持续时间的不同,GOPs的丰富程度有所不同.
- 通过诊断碎片离子,HRMS/MS分析证实了GOPs的身份,显示了不同的形成模式.
结论:
- 冷气等离子体在临床相关的葡萄糖水平上有效地产生GOPs的光谱.
- 已识别的GOPs,其中许多具有已知的细胞毒性或信号作用,阐明了氧化环境中的葡萄糖氧化机制.
- 基于等离子体的氧化模型是研究GOP相关的生物效应和病理生理影响的宝贵工具.
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