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转录组分析揭示了肝中脂质新陈代谢和免疫功能的循环节律调节

Jiahua Li^1,2, Jie Dong^1,2, Minjie Huang^1,2

¹Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.

Animals : an open access journal from MDPI

|November 27, 2025

概括

肝节律显著影响脂质代谢和免疫功能. 这项研究确定了关键的节律基因和通路,揭示了免疫和脂质过程中明显的明暗相活动.

科学领域:

* 时间生物学和家禽科学.
* 鸟类肝脏中昼夜节律的分子机制.
* 生物节奏,脂质代谢和免疫功能之间的相互作用.

背景情况:

*肝脏的昼夜节律会影响的关键生理过程,包括脂质代谢和免疫反应.
*关于鸟类肝脏中控制这些节律功能的特定基因和分子通路的了解有限.
*研究这些节奏对于优化家禽健康,抗病能力和生产效率至关重要.

研究的目的:

* 为了阐明肝脏节律的转录形态在的光/黑暗循环.
* 确定参与节律性脂质代谢和免疫功能的关键基因和途径.
* 了解肝中明暗阶段之间的基因表达模式差异.

主要方法:

* 在24小时内在7个时间点收集的肝样本的转录组测序.
*趋势分析以确定节律性基因表达模式 (概况2和5).
*权重基因共同表达网络分析 (WGCNA) 和Cosinor分析,以确定节律相关的基因模块和特定的节律表达基因.

主要成果:

*确定了与免疫功能 (例如FAM21C,TLR2A) 和脂质代谢 (例如MSMO1,ELOVL2) 相关的基因的节奏表达.
* 检测到845个在光明和黑暗条件之间不同表达的基因,脂质代谢和免疫功能发生显著变化.
*像自这样的免疫过程在光明阶段更活跃,而固醇生物合成在黑暗阶段更活跃. 确定了与肝脏昼夜节律相关的关键基因模块 (棕色和蓝色).
* 在9个与脂质相关的和11个与免疫相关的基因中确认了显著的节律表达.

结论:

* 的肝免疫功能和脂质代谢表现出显著的日常节律.
* 特定的基因和通路是节律调节的,在光明和黑暗阶段影响着不同的生理活动.
* 结果提供了对疾病抵抗和脂质沉积的调节的关键见解,为改善家禽健康和生产率提供了目标.

关键词:

这些都是肉肉.肝脏节律肝脏节律免疫系统是免疫系统的.脂质新陈代谢脂质新陈代谢转录组 (transcriptome) 是一个转录组.

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