莱沃布皮瓦卡因的使用抑制了人类腺癌A549细胞的细胞代谢
Masae Iwasaki1, Makiko Yamamoto1, Masahiro Tomihari1
1Department of Anesthesiology and Pain Medicine, Graduate School of Medicine, Nippon Medical School, 1-1-5, Sendagi, Bunkyo 113-8602, Tokyo, Japan.
International journal of molecular sciences
|November 27, 2025
概括
利沃布皮瓦卡因通过减少ATP合成来抑制肺腺癌细胞代谢. 这种抗癌效应取决于血管激素转化酶2 (ACE2) 的表达.
科学领域:
- 在瘤学瘤学.
- 麻醉学 麻醉学
- 分子生物学分子生物学
背景情况:
- 在外科手术期间使用的麻醉剂可能会影响癌细胞生物学.
- 肺腺癌是全球重要的健康问题.
研究的目的:
- 为了研究levobupivacaine对肺腺癌 (A549) 细胞的影响.
- 阐明血管酶转化酶2 (ACE2) 在调解levobupivacaine作用中的作用.
主要方法:
- 用levobupivacaine (0.1mM, 0.5mM) 治疗A549细胞,有或没有ACE2 siRNA.
- 细胞增殖 (CCK-8试验),ATP合成 (CellTiter-Glo试验) 和基因表达 (RT-PCR) 被评估.
- 分析了包括HIF-1α,MMP-9,β-catenin,BCL2和WNT1在内的关键生物标志物.
主要成果:
- 利沃布皮瓦卡因抑制了ATP合成和细胞代谢,但没有影响增殖.
- 观察到ACE2的上调和促癌生物标志物 (HIF-1α,MMP-9,β-catenin,BCL2,WNT1) 的下调.
- 莱沃布皮瓦卡因的抗癌作用依赖于ACE2表达,正如siRNA实验所证明的那样.
结论:
- 莱沃布皮瓦卡因通过抑制新陈代谢,对肺腺癌细胞表现出抗癌特性.
- 观察到的效应通过一种依赖于ACE2的途径进行介导.
- 麻醉选择可能需要在术后癌症护理中考虑.
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