在调节THP-1单细胞迁移和MCP-1-诱导的炎症反应中CD147的双重作用
Nutjeera Intasai1,2, Kanokporn Sornsuwan2,3, On-Anong Juntit2,3
1Division of Clinical Microscopy, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.
International journal of molecular sciences
|November 27, 2025
概括
差异化集群 (CD) 147个淘汰单细胞显示出增加的迁移和改变的炎症性细胞因子生产. 这表明CD147调节单细胞化学反应和炎症平衡.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
背景情况:
- 差异化集群 (CD) 147是一种跨膜糖蛋白,对单细胞功能至关重要,如入侵,迁移和细胞因子生产.
- 单细胞化学吸引蛋白-1 (MCP-1) 是一个关键的化学因子,参与单细胞招募和炎症反应.
研究的目的:
- 研究CD147在调节单细胞化学反应和炎症反应中的作用.
- 分析CD147淘汰赛 (CD147KO) 对THP-1单细胞迁移和细胞因子概况对MCP-1的反应的影响.
主要方法:
- 使用了CD147淘汰赛 (CD147KO) THP-1单细胞和野生型 (WT) THP-1细胞.
- 评估单细胞向MCP-1和乳腺癌细胞分泌的化学吸引剂的迁移.
- CD44的量化表面表达,CC化学因子受体1型 (CCR1) 和CC化学因子受体2型 (CCR2).
- 在MCP-1刺激后测量了关键炎性细胞因子 (IL-6,IL-10,TNF-α) 的mRNA表达.
主要成果:
- 与WT细胞相比,CD147KO THP-1细胞的迁移显著增强.
- CD44,CCR1和CCR2的表面表达在CD147KO细胞中保持不变.
- 刺激MCP-1导致IL-6和IL-10mRNA表达升高,并在CD147KO单细胞中减少TNF-αmRNA表达.
- 鉴定出IL-6上调作为增强单细胞迁移的潜在媒介.
结论:
- CD147作为THP-1单细胞迁移的监管检查点,其功能取决于上下文.
- CD147淘汰会影响单细胞中促炎和抗炎细胞因子反应之间的平衡.
- 这些发现提供了关于CD147在免疫细胞行为和炎症过程中的复杂作用的见解.
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