开发RNA Aptamers,以抑制SARS-CoV-2菌株的RNA依赖RNA聚合酶活性在体外
Chaewon Song1, Seong-Wook Lee1,2
1Department of Bioconvergence Engineering, Research Institute of Advanced Omics, Dankook University, Yongin 16890, Republic of Korea.
International journal of molecular sciences
|November 27, 2025
概括
研究人员开发了针对保存的SARS-CoV-2RNA依赖RNA聚合酶 (NSP12) 的新型RNA吸附体. 这些体有效地抑制了多种变体的病毒复制,提供了潜在的广泛的抗病毒策略.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 药物发现 药物发现 药物发现
背景情况:
- 新出现的SARS-CoV-2变种需要突变独立的抗病毒策略.
- 病毒RNA依赖RNA聚合酶 (NSP12) 是治疗干预的保存和基本点.
研究的目的:
- 开发针对高度保守的SARS-CoV-2 NSP12的RNA吸收体.
- 为了评估这些体的有效性作为各种SARS-CoV-2菌株的宽谱抑制剂.
主要方法:
- 通过指数丰富的连接体的系统演化 (SELEX) 用于识别针对NSP12的向体.
- RNA-蛋白 pull-down 和竞争试验证实了阿胺酶结合的亲和力和特异性.
- 在实验室初级扩展试验中,评估了对NSP12RNA依赖RNA聚合酶 (RdRp) 活性的aptamer的抑制活性.
主要成果:
- 成功识别了针对NSP12的高亲和度RNA吸收体.
- 选择的阿马体在体外表现出有效抑制NSP12 RdRp活性.
- 阿普塔默在野生型,阿尔法,三角形和奥米克朗SARS-CoV-2变种中显示出一致的结合和抑制.
结论:
- 开发的RNA吸附体显示出作为SARS-CoV-2的广谱抑制剂的潜力.
- 这些阿普坦体向NSP12的保护区域,使它们对当前和新兴变异有效.
- 这些发现表明,开发针对SARS-CoV-2和其他RNA病毒的新型抗病毒剂是一个有希望的平台.
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