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基于PCR的分子身份验证方法,通过对完整的叶绿体基因组进行比较分析来验证农业草原的来源

Woojong Jang¹, Sae Hyun Lee², Wook Jin Kim¹

¹Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, Naju 58245, Republic of Korea.

International journal of molecular sciences

|November 27, 2025

概括

准确的物种识别对于草药质量至关重要. 这项研究从叶绿体基因组中开发了新的DNA标记,可靠地区分四种Agrimonia物种,确保Agrimoniae Herba.的真实性.

科学领域:

基因组学就是基因组学.
分子生物学分子生物学
药理学是指药理学,即药理学是指药理学.

背景情况:

精确的物种识别对于草药质量控制至关重要.
对*Agrimonia*物种的有限基因组数据阻碍了分子识别方法.
对于Agrimoniae Herba来说,传统上使用*Agrimonia*物种需要可靠的认证.

研究的目的:

为了组装四种 *Agrimonia * 种类的完整的叶绿体基因组.
为了确定特定物种的变异,开发分子标记物.
建立可靠的分子工具,用于*Agrimonia*物种认证.

主要方法:

测序和组装四个完整的叶绿体基因组.
对比基因组分析以识别单核酸多态 (SNP) 和插入/删除 (InDels).
基于PCR的分子标记物的开发和验证,使用基因组变异和对标本和产品的测试.

主要成果:

组装了四种*Agrimonia*物种的完整叶绿体基因组,具有典型的四部分结构和112个独特的基因.
确定了684个变量站点,包括497个SNP和187个InDels,主要是在单副本地区.
九个经过验证的基于PCR的标记物成功区分了四种物种,显示了实际适用性.

结论:

叶绿体基因组序列提供了有效的超级DNA条形码,用于识别*Agrimonia*物种.
开发的分子工具提供了可靠的方法来验证Agrimoniae Herba的真实性.
这项研究提供了基本的基因组资源和认证工具,支持草药的安全性和有效性.

关键词:

亚格里莫尼亚农业公司农业植物草 (Agrimoniae Herba) 是一种植物.叶绿体基因组的基因组分子身份验证标记器分子身份验证标记器标识物种标识物种标识物种

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