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铁氨酸激酶在精子容量中调解EGFR激活

Odeya Yemini-Talbi¹, Uri Nir¹, Haim Breitbart¹

¹The Mina & Everard Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 5290002, Israel.

International journal of molecular sciences

|November 27, 2025

概括

铁氨酸激酶对于哺乳动物的精子受精和适当的体反应 (AR) 至关重要. 它的抑制会损害体外受精 (IVF) 率,并通过影响表皮生长因子受体 (EGFR) 激活来破坏精子容量.

科学领域:

生殖生物学生殖生物学
分子细胞生物学分子细胞生物学
生物化学生物化学

背景情况:

精子电容和体反应 (AR) 对于受精至关重要.
在卵细胞遇到前发生的自发性体反应 (SAR) 会降低精子受精能力.
铁氨酸激酶在精子功能中的作用仍然不完全理解.

研究的目的:

调查铁氨酸激酶在哺乳动物精子电容和受精中的作用.
阐明在精子功能期间涉及铁,表皮生长因子受体 (EGFR) 和通道的信号通路.

主要方法:

在小鼠精子中抑制铁氨酸激酶活性.
在体外受精 (IVF) 率的评估.
对EGFR激活和SAR发生率的分析.
通过CatSper通道调查 (Ca2+) 流入.
探索涉及蛋白激酶A (PKA) 的信号级联.

主要成果:

铁的抑制使试管婴儿率降低了75%,并增加了SAR的发生率.
在电容化期间的铁抑制降低了EGFR激活,但在AR期间没有.
铁对EGFR和SAR的影响通过CatSper Ca2+通道进行介导.
CatSper抑制增加了铁的激活,而高细胞内Ca2+抑制了它.
一个信号通路 (HCO3− → SAC → cAMP → PKA → Src → Fer → EGFR) 调节铁介导EGFR的激活.

结论:

铁氨酸激酶在精子电容和受精中发挥着关键作用.
铁在电容化过程中调节EGFR激活,影响SAR和受精能力.
涉及PKA,CatSper和Fer的新型信号级联控制精子中的EGFR激活.

关键词:

欧洲农业基金会 (EGFR) 是一个.一个宏体的宏体.电容容量定位电容容量定位容量定位铁酶是铁酶的一种.的反应反应反应反应反应.精子精子精子

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