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益生菌中氧酸盐降解酶的生物信息分析:一个系统的基因组规模和结构调查

Shengda Du¹, Ke Sun², Bo Xiao³

¹College of Life Sciences, Northwest A&F University, Yangling 712100, China.

|November 27, 2025

概括

这项研究确定了具有氧酸盐降解酶的益生菌菌株,以对抗结石. 这些发现引导了开发新的益生菌来治疗氧化酸 urolithiasis.

科学领域:

生物信息学是一种生物信息学.
微生物学微生物学
泌尿器科泌尿器科泌尿器科泌尿器科

背景情况:

结石疾病,主要是氧酸盐沉,在全球的发病率正在增加.
目前治疗结石的疗法具有可变的疗效和高的复发率.

研究的目的:

从理论上评估益生菌物种的氧酸盐降解潜力.
为了确定益生菌候选人开发功能性益生菌来管理结石.

主要方法:

使用BLASTp的生物信息分析对38种益生菌物种进行同质性搜索.
确定关键的氧酸盐降解基因 (OXC,FRC,OXDC,OOR).
结构预测使用AlphaFold和TM-align对保存的酶折叠的评分.

主要成果:

鉴定了7种与合氧基-CoA脱碳酶 (OXC) 和甲基-CoA转移酶 (FRC) 途径结合的菌株.
一个菌株被鉴定为含有氧酸盐脱碳酶 (OXDC),三种菌株被鉴定为含有氧酸盐氧化还原酶 (OOR).
高的TM分数 (>0.8) 表明酶结构保存,表明功能性酸盐降解.

结论:

这项研究为选择具有氧酸盐降解能力的益生菌提供了理论基础.
已识别的菌株作为下一代功能益生菌的候选者,可缓解结石疾病.

关键词:

阿尔法折叠是什么意思阿尔法折叠在TM-align.酶是一种酶.结石疾病结石疾病氧沙酸盐降解降解的方法益生菌是一种益生菌.

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