一个与内和种子特征相关的米GH5_11基因的表征
Koen Gistelinck1, Zoë Madder1, Isabel Verbeke1
1Laboratory of Biochemistry and Glycobiology, Department of Biotechnology, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, Belgium.
Plants (Basel, Switzerland)
|November 27, 2025
概括
研究人员研究了大米中的糖化物化酶 (GHs),确定了17个GH5基因. GH5_11基因LOC_Os04g40510影响种子的发育和生育能力,影响内精和种子组.
科学领域:
- 植物生物学 植物生物学
- 生物化学 生物化学
- 遗传学 遗传学 是一个
背景情况:
- 植物细胞壁对于结构和生理调节至关重要.
- 细胞壁的动力学涉及细胞壁修饰酶,特别是甘氨酸酸酶 (GHs).
- GH5家族是具有多种功能的GHs的一个重要群体.
研究的目的:
- 在大米 (Oryza sativa L.) 中识别和分类GH5家族成员.
- 在大米中描述特定GH5_11基因LOC_Os04g40510的功能.
- 研究GH5_11酶在种子发育和生育中的作用.
主要方法:
- 生物信息分析用于识别大米基因组中的GH5基因.
- 使用GFP标记蛋白用于LOC_Os04g40510.10的亚细胞定位.
- 转基因大米在发芽过程中的基因表达分析和表型评估.
主要成果:
- 在大米中发现了17个GH5基因,分为GH5_7,GH5_11和GH5_14亚家族.
- 由LOC_Os04g40510编码的GH5_11蛋白通过分泌途径合成,并在种子中表达,特别是内.
- 过度表达LOC_Os04g40510导致种子形态发生变化,度增加,种子数量减少;倒线显示种子质量减少.
结论:
- LOC_Os04g40510 在米的生育能力和内的发育中起着重要的作用.
- GH5_11酶参与调节的种子特性和繁殖成功.
- 这项研究为大米中GH5_11酶的生物功能提供了新的见解.
更多相关视频
07:43Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice
Published on: October 6, 2020
13.2K
07:34Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function
Published on: May 5, 2023
4.9K
相关概念视频
01:59Plant Breeding and Biotechnology
21.4K
Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
21.4K
02:27Trihybrid Crosses
25.2K
Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal...
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal...
25.2K
02:50Transgenic Plants
8.4K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
8.4K
01:20Monohybrid Crosses
238.7K
Overview
238.7K
01:25Cell Signaling in Plants
6.1K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
6.1K
01:18Dihybrid Crosses
80.8K
Overview
80.8K