染色素相关的豆酸酶psNTP9 作为酵母和阿拉比多普西斯的DNA结合调节蛋白的功能
Huan Wang1, Robert D Slocum2, Xingbo Cai1
1Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX 78712, USA.
Plants (Basel, Switzerland)
|November 27, 2025
概括
Pea apyrase (PS) 和它的修饰形式 (DM) 结合于酵母和Arabidopsis中的不同的DNA促进子区域. 这种DNA结合能力表明植物酶在调节跨物种基因表达方面发挥了新的作用.
科学领域:
- 分子生物学分子生物学
- 植物科学 植物科学
- 酵母遗传学 酵母遗传学
背景情况:
- 之前已经表明,豆酸酶 (PS) 和一个修改版 (DM) 能够局部化到酵母核中,并结合非重叠的促进子区域.
- 这些蛋白质在酵母中表现出不同的基因表达特征,与观察到的表型相关.
研究的目的:
- 为了研究PS和DM在Arabidopsis中的DNA结合活性.
- 在酵母和植物系统中描述这些植物apyrases的结合特异性和潜在的基因调控功能.
主要方法:
- 染色体免疫沉测序 (ChIP-seq) 用于识别Arabidopsis中的DNA结合位点.
- 电泳运动转移试验 (EMSA) 来验证DNA结合特异性.
- 用AlphaFold3建模来预测蛋白质-DNA相互作用和结合动机.
主要成果:
- PS和DM与Arabidopsis中大致不同的促进子区域有关,反映了酵母结合模式.
- 聚氨酸表现出对酵母和植物促进剂序列的特定结合,而DM结合是最小的.
- 通过AlphaFold3建模,确定了PS.的潜在DNA结合残留物和保存的基因 (5'-(G/T) GGG(G/T) A-3').
结论:
- 像PS这样的植物apyrases可以作为能够调节基因表达的DNA结合蛋白发挥作用.
- 这些发现扩大了已知的植物酶的功能,超出了戈尔吉或细胞外基质.
- 这项研究支持PS在酵母和阿拉比多普西斯的基因调节中的保留作用.
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