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基于转录组的miR156-中介的SPL转录因子表达动态在菜中植物性到繁殖性过渡期间
Ehsan Khalid1,2, Yutong Zheng2, Tengqi Wang2
1Corp Genetics and Breeding, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
菜的miR156微RNA通过调节SPL转录因子来控制植物的发育. 这项研究揭示了miR156如何准SPL基因,以管理从植物生长到开花的关键过渡.
科学领域:
- 植物分子生物学 植物分子生物学
- 发展生物学 发展生物学
- 遗传学 是一个遗传学.
背景情况:
- 微RNAs (miRNAs) 是基因表达的关键调节者,影响植物发育.
- 调节模块miR156-SPL控制植物从植被到繁殖阶段的过渡,但其在菜中的特点尚不清楚.
研究的目的:
- 调查miR156及其目标SPL转录因子在菜阶段过渡中的作用.
- 确定菜中的SPL基因成员,并通过SpmiR156.6分析它们的调节.
主要方法:
- 在菜中发现SPL基因的全基因组识别.
- 转录组分析以确定miR156的目标.
- 基于序列的相互作用预测.
- 使用定量实时聚合酶链反应 (qRT-PCR) 进行表达特征分析.
- 基因本体学 (GO) 和促进者cis元素分析.
主要成果:
- 在菜中确定了14个SPL基因成员.
- 发现11个SPL基因可能是SpmiR156a/b/c/d的直接目标.
- 在过渡阶段,SpSPL3和SpSPL8显示出显著的调节.
- SPL基因与荷尔蒙路径和花发育有关.
结论:
- 在菜中,SpmiR156在调节植物向生殖阶段过渡方面发挥着核心作用.
- 这项研究阐明了涉及SpmiR156和SpSPL基因用于发育阶段变化的监管框架.
- 强调了菜中SPL基因的扩张和功能多样化.
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