Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Leaky Scanning02:28

Leaky Scanning

5.6K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.6K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Prevalence of celiac disease among permanent residents in Northwest China: A cross-sectional survey.

Scientific reports·2026
Same author

The plasma metabolome and clinical features of patients with coeliac disease in Northwest China.

Annals of medicine·2026
Same author

[A rapid visual detection method for porcine circovirus type 4 based on enzymatic recombinase amplification and CRISPR/<i>Es</i>Cas13d].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology·2026
Same author

Integrative computational elucidation of molecular mechanisms and multi-target interactions in paraquat-induced pulmonary fibrosis.

Toxicology and industrial health·2026
Same author

Microplastic exposure and human health risks across the life cycle: a focus on reproduction, development, and aging.

Frontiers in cell and developmental biology·2026
Same author

Biological processes dominate the dynamic changes in the dissolved inorganic carbon ion equilibrium system of coastal wetlands under tidal influence.

Marine environmental research·2026

相关实验视频

Updated: Jan 10, 2026

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens
10:19

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens

Published on: February 3, 2020

6.8K

转录学揭示了斯库特拉林对感染PRRSV的PAMs的抑制作用.

Guidong Zhang1, Teng Tu2, Yanwei Li1

  • 1College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.

Viruses
|November 27, 2025
PubMed
概括

斯库特拉林 (SCU) 有效地抑制了猪生殖和呼吸系统综合征病毒 (PRRSV) 在猪中的感染. 这种天然化合物直接向病毒,并可能通过调节宿主免疫和炎症途径来起作用.

关键词:
猪生殖和呼吸系统综合征病毒这样做,你就会发现自己是什么样子的.转录组分析 转录组分析

更多相关视频

Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression
10:48

Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression

Published on: May 15, 2014

11.8K
Using Click Chemistry to Measure the Effect of Viral Infection on Host-Cell RNA Synthesis
09:35

Using Click Chemistry to Measure the Effect of Viral Infection on Host-Cell RNA Synthesis

Published on: August 9, 2013

12.2K

相关实验视频

Last Updated: Jan 10, 2026

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens
10:19

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens

Published on: February 3, 2020

6.8K
Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression
10:48

Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression

Published on: May 15, 2014

11.8K
Using Click Chemistry to Measure the Effect of Viral Infection on Host-Cell RNA Synthesis
09:35

Using Click Chemistry to Measure the Effect of Viral Infection on Host-Cell RNA Synthesis

Published on: August 9, 2013

12.2K

科学领域:

  • 兽医病毒学 兽医病毒学
  • 自然产品化学 自然产品化学
  • 免疫学 免疫学 免疫学

背景情况:

  • 猪生殖和呼吸系统综合征 (PRRS) 是由猪生殖和呼吸系统综合征病毒 (PRRSV) 引起的全球猪业重大威胁.
  • 高的PRRSV突变率降低了疫苗的疗效,需要开发新的抗病毒药物.
  • 斯库特拉林 (SCU) 是一种来自*Scutellaria baicalensis*的黄类物质,已知具有抗病毒性质,但其对PRRSV的影响尚未研究.

研究的目的:

  • 对猪生殖和呼吸系统综合征病毒 (PRRSV) 的抗病毒活性进行调查.
  • 为了阐明SCU在体外对PRRSV感染的作用机制.

主要方法:

  • 在体外细胞培养实验中使用感染PRRSV的猪膜巨细胞 (PAMs) 进行细胞培养实验.
  • 转录组分析以确定受SCU治疗影响的宿主通路.
  • 对SCU针对PRRSV的直接抗病毒活性测定.

主要成果:

  • 斯库特拉林在PAM中显著抑制了PRRSV感染.
  • 发现SCU直接作用于PRRSV以达到抗病毒效果.
  • 转录组数据表明,SCU通过补充和凝血级联,ECM受体相互作用,Rap1和PI3K-Akt信号传递等途径调节宿主免疫和抗炎作用.

结论:

  • 斯库特拉林对PRRSV具有强大的抗病毒活性.
  • SCU的机制涉及直接的病毒抑制和调节宿主免疫和炎症反应.
  • SCU代表了管理猪中PRRSV感染的潜在治疗候选者.