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从乳酸细菌中制备的溶解的酸甘油衍生物的免疫刺激活性

Sho Noguchi¹, Momoko Nakayama², Masahiro Eguchi²

¹Graduate School of Engineering, Kanto Gakuin University, 1-50-1 Mutsuura-higashi Kanazawa-ku Yokohama, Kanagawa, Japan.

Microbiology and immunology

|November 27, 2025

概括

来自Levilactobacillus brevis和Lactiplantibacillus plantarum的细菌酸糖 (PG) 显示出作为疫苗辅助剂的潜力. 这些PG制剂刺激免疫细胞,表明它们在增强疫苗疗效方面的有用性.

科学领域:

免疫学免疫学免疫学
微生物学微生物学
生物化学生物化学

背景情况:

细菌糖素 (PG) 是细菌细胞壁的关键成分,具有已知的免疫刺激特性.
开发新型疫苗辅助剂对于提高疫苗有效性和克服疫苗设计方面的挑战至关重要.

研究的目的:

为了研究从莱维拉克托巴塞勒斯 (Levilactobacillus brevis) 和乳植物菌 (Lactiplantibacillus plantarum) 中溶解于溶酶的PG作为疫苗辅助剂的潜力.
描述这些PG制剂的免疫刺激活性,并确定对其活性负责的结构成分.

主要方法:

来自L. brevis和L. plantarum的PG的酶溶解.
在THP-1细胞中对IL-8诱导的评估.
定期氧化PG以调查muramyl二的作用.
在NOD1和NOD2表达细胞中对IL-8诱导的评估.
使用RAW264.7细胞进行的增殖试验.

主要成果:

来自两种细菌物种的溶解PG诱导了THP-1细胞中的IL-8产生.
定期氧化L. plantarumPG降低了IL-8的诱导,这表明muramyl dipeptide的部分破坏.
周期性氧化L. plantarumPG在NOD2表达细胞中的活性降低,但在NOD1表达细胞中保持活性,这表明维护了γ-d-glutamyl-meso-diaminopimelic酸结构.
所有PG制剂都刺激了RAW264.7细胞增殖.

结论:

来自L. brevis和L. plantarum的酶溶解PG表现出强大的免疫刺激活性.
聚烯的结构完整性,特别是 γ-d-glutamyl-meso-diaminopimelic 酸成分,对于其与 NOD1.1 的相互作用很重要.
这些PG制剂是作为疫苗辅助剂开发的有希望的候选者.

关键词:

一个IL-8的IL-8.乳酸细菌是一种乳酸细菌.这是一种Muramyl二化物.酸甘油可以是酸甘油.

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