在没有外部电子捐赠者的废物活性泥中,通过定向微生物水解启动Caproate生产
Jun-Yan Liu1, Kun Dai1, Qing-Song Lin1
1Center of Wastewater Resource Recovery, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China.
Environmental science & technology
|November 27, 2025
概括
这项研究介绍了一种富含微生物联盟,该联盟从废弃活性污泥 (WAS) 产生中链脂肪酸 (MCFA),无需外部电子捐赠者. 这种方法提高了从污泥中回收的MCFA.
科学领域:
- 环境微生物学 环境微生物学
- 生物技术是生物技术.
- 废物管理 废物管理
背景情况:
- 由于产量低,废弃活性污泥 (WAS) 的中链脂肪酸 (MCFA) 生产有限.
- 目前的方法通常需要外部电子捐赠者和预处理,增加成本和复杂性.
研究的目的:
- 开发一种方法,可以在没有外部电子捐赠者的情况下直接从WAS启动MCFA生产.
- 为了确定微生物联盟和代谢途径,负责增强的MCFA生产.
主要方法:
- 使用了一个缩的MCFA生产联盟 (MPC) 接种到WAS.
- 采用多组学分析来识别关键细菌 (例如,Bacteroides) 和代谢途径 (例如,脂肪酸生物合成).
- 量化化学氧需求 (COD) 转换为短链脂肪酸 (SCFA) 和MCFA.
主要成果:
- 与单独使用WAS相比,MPC显著提高了COD转换率为SCFA (6.0%至20.7%) 和MCFA (0%至4.6%).
- MPC表现出更高的水解活性,特别是酸酶,对于降解尿酸至关重要.
- 脂肪酸生物合成 (FAB) 途径和相关的基因/酶被确定为MCFA转换的核心.
结论:
- 使用MPC的定向微生物方法可以在没有外部电子捐赠者的情况下从WAS直接产生MCFA.
- 该过程依赖于增强的水解活性和脂肪酸生物合成途径.
- 这种方法为从废物污泥中回收有价值的MCFA提供了一个有希望的策略.
更多相关视频
15:19Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor
Published on: October 15, 2015
10.1K
11:58Waste Water Derived Electroactive Microbial Biofilms: Growth, Maintenance, and Basic Characterization
Published on: December 29, 2013
14.0K
相关概念视频
Amino Acid Catabolism
952
Microorganisms rely on proteins as an essential carbon and energy source, particularly in environments with limited polysaccharides or lipids. However, proteins are too large to cross the plasma membrane unaided, necessitating enzymatic degradation. Microbes secrete extracellular proteases and peptidases that hydrolyze proteins into peptides, which can then be transported across the membrane. Once inside the cell, intracellular proteases degrade these peptides into free amino acids, which...
952
Carbon-dioxide Fixation
597
Carbon dioxide fixation in prokaryotes enables the assimilation of inorganic carbon into organic molecules, supporting biosynthetic pathways, sustaining ecosystems, and contributing to the global carbon cycle. It also has industrial applications in carbon capture and bioproduct synthesis. Autotrophic organisms rely on this process to utilize CO₂ as a carbon source in diverse environments.The Calvin CycleThe Calvin cycle is the most widespread carbon fixation mechanism, primarily used by...
597
Fates of Pyruvate
10.4K
Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
10.4K
Microbial Fermentation
1.3K
Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
1.3K
Metabolism of Chemolithotrophs
728
Chemolithotrophs are microorganisms that obtain energy by oxidizing inorganic molecules such as hydrogen gas (H₂), ammonia (NH₃), reduced sulfur compounds (H₂S, S²⁻), and ferrous iron (Fe²⁺). Unlike heterotrophic organisms that rely on organic carbon, chemolithotrophs transfer electrons from these inorganic donors to the electron transport chain (ETC), generating a proton motive force (PMF) that drives ATP synthesis through oxidative phosphorylation.
728
Lipid Catabolism
822
Triglycerides serve as crucial long-term energy storage molecules in microorganisms, providing a dense source of metabolic energy. Their breakdown is mediated by lipases, which hydrolyze triglycerides into glycerol and free fatty acids. Each of these components follows distinct metabolic pathways, ultimately contributing to ATP synthesis and cellular energy homeostasis.Glycerol MetabolismGlycerol, released from triglyceride hydrolysis, is phosphorylated by glycerol kinase to form...
822
