在人类大脑器官中神经发生过程中表征组织氧气张力
Yuan-Hsuan Liu1, Hsiao-Mei Wu1,2,3
1Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan.
Bio-protocol
|November 27, 2025
概括
在3D人类大脑器官 (hCOs) 中量化氧气具有挑战性. 这项研究引入了一种使用嵌入式微珠和先进显微镜的新方案,以精确测量神经发生过程中的氧气动态.
科学领域:
- 神经科学是一个神经科学.
- 生物医学工程 生物医学工程
- 生物物理学的生物物理.
背景情况:
- 氧气压力极大地影响了人类早期的神经发生.
- 在3D有机体模型中长时间测量组织内氧气的准确,不干扰的方法缺乏.
- 目前的技术,如微传感器或高刺激探头限制测量时间或干扰有机体.
研究的目的:
- 为测量人类大脑器官 (hCOs) 内器官内氧水平提出详细的协议.
- 为了实现与神经发育过程相关的氧气动态的纵向跟踪.
- 在复杂的3D生物模型中克服现有的氧气测量技术的局限性.
主要方法:
- 使用嵌入在人类大脑器官 (hCOs) 中的基于的CPOx微珠.
- 采用广场频域光终身成像显微镜 (FD-FLIM) 进行氧气传感.
- 开发了一个端到端的工作流程,包括有机体图案,珠子共嵌入,标准化的FD-FLIM获取,MATLAB的自动数据分析和斯特恩-沃尔默校准.
主要成果:
- 在单个hCO中生成每珠氧气图和纵向氧气图案.
- 基于珠子位置 (器官内与凝) 和样本状态 (健康与异常) 的分层氧气动态.
- 证明了将发育成长和氧气动态在珠子水平上联系起来的能力.
结论:
- 本协议提供了一个强大的,不扰乱的方法,用于hCOs的纵向氧张力测量.
- 这种技术有助于更深入地了解氧气在人类神经发生和有机体发育中的作用.
- 该工作流为研究人员研究3D有机体模型提供可复制,即时使用的工具.
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