Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Mechanism of Filopodia Formation01:39

Mechanism of Filopodia Formation

3.0K
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
3.0K
Cell Polarization by Rho Proteins01:21

Cell Polarization by Rho Proteins

3.5K
Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
3.5K
Cell Motility through Blebbing01:16

Cell Motility through Blebbing

2.4K
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
2.4K
Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

3.5K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
3.5K
Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

3.4K
In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...
3.4K
Notch Signaling Pathway03:14

Notch Signaling Pathway

6.4K
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
6.4K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

The association between the Acute Physiology Score and sepsis in ICU non-surviving patients with inflammatory bowel disease: a retrospective cohort study from the eICU-CRD.

Frontiers in medicine·2026
Same author

Saikosaponin A restores the IDO1-driven gut-testis kynurenine axis to alleviate oligozoospermia.

Phytomedicine : international journal of phytotherapy and phytopharmacology·2026
Same author

Developmental Trajectories of Problematic Gaming among Chinese Migrant Children: Predictors and School Adjustment Outcomes.

Journal of youth and adolescence·2026
Same author

Gut microbiota-derived Tryptophanol driven by N-Carbamylglutamate alleviates premature ovarian failure through inhibiting oxidative stress.

Journal of ovarian research·2026
Same author

Epidemiological characteristics and transmission dynamics of COVID-19 outbreaks in China: a four-year retrospective analysis using extended SEIR models.

BMC public health·2026
Same author

Obesity impairs spermatogenesis via Leydig cell ferroptosis induced by liver-derived exosomal miR-122-5p.

Communications biology·2026

相关实验视频

Updated: Jan 10, 2026

Single Cell Durotaxis Assay for Assessing Mechanical Control of Cellular Movement and Related Signaling Events
08:30

Single Cell Durotaxis Assay for Assessing Mechanical Control of Cellular Movement and Related Signaling Events

Published on: August 27, 2019

8.4K

包含的刚度感知指导差异化的细胞延长和力量生成跨现象型.

Yuxin Luo1, Yimin Luo1

  • 1Department of Mechanical Engineering, Yale University, New Haven, Connecticut 06511, United States.

ACS biomaterials science & engineering
|November 27, 2025
PubMed
概括
此摘要是机器生成的。

3D原体中的硬微凝引导纤维细胞和肌纤维细胞的行为,揭示了一个反循环,在这种循环中,局部刚性维持了纤维肌纤维细胞表型. 这突显了机械的异质性.

关键词:
生物材料是一种生物材料.这种水凝是水凝.机械传导 机械传导肌纤维母细胞细胞.两极分化是一种极化.改造 改造 改造 改造 改造 改造

更多相关视频

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers
09:56

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

Published on: August 31, 2021

5.5K
Live Cell Imaging of Microtubule Cytoskeleton and Micromechanical Manipulation of the Arabidopsis Shoot Apical Meristem
07:52

Live Cell Imaging of Microtubule Cytoskeleton and Micromechanical Manipulation of the Arabidopsis Shoot Apical Meristem

Published on: May 23, 2020

5.8K

相关实验视频

Last Updated: Jan 10, 2026

Single Cell Durotaxis Assay for Assessing Mechanical Control of Cellular Movement and Related Signaling Events
08:30

Single Cell Durotaxis Assay for Assessing Mechanical Control of Cellular Movement and Related Signaling Events

Published on: August 27, 2019

8.4K
Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers
09:56

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

Published on: August 31, 2021

5.5K
Live Cell Imaging of Microtubule Cytoskeleton and Micromechanical Manipulation of the Arabidopsis Shoot Apical Meristem
07:52

Live Cell Imaging of Microtubule Cytoskeleton and Micromechanical Manipulation of the Arabidopsis Shoot Apical Meristem

Published on: May 23, 2020

5.8K

科学领域:

  • 生物材料科学 生物材料科学
  • 细胞生物学 细胞生物学
  • 机械生物学 机械生物学

背景情况:

  • 纤维化涉及纤维细胞到肌纤维细胞的过渡,受细胞外矩阵力学的影响.
  • 当前的研究往往忽视了3D环境和机械异质性.
  • 在3D中了解局部刚性对细胞行为的影响对于纤维化研究至关重要.

研究的目的:

  • 研究3D环境中的局部矩阵刚性如何差异调节纤维细胞和肌纤维细胞的行为.
  • 探索机械异质性在细胞极化,收缩和表型维护中的作用.
  • 通过机械线索来发现调节纤维化进展的潜在机制.

主要方法:

  • 工程3D微凝结合物复合材料,具有不同的微凝刚性.
  • 嵌入的细胞尺度微凝 (软/硬) 在一个原基质.
  • 在对微凝的反应中分析了细胞极化,力生成和表型标记物定位 (α-SMA,YAP).

主要成果:

  • 纤维细胞和肌纤维细胞都以距离依赖的方式偏向微凝内含物.
  • 肌纤维细胞表现出较慢的极化衰变,表明对机械变化的敏感性更高.
  • 坚硬的微凝促进了肌纤维细胞表型的稳定性和增强的力量产生,与软的微凝或纯原不同.

结论:

  • 一个现象型和刚性依赖的反循环存在:硬的入驱动肌纤维细胞极化和原重塑,加强肌纤维细胞现象型.
  • 机械异质性作为纤维化过程中细胞行为的关键调节者.
  • 这项研究提供了关于调节纤维化的见解,通过准细胞外基质中的机械线索来调节纤维化.