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编码在 JC 聚瘤病毒中的 miRNA jcv-miR-J1-5p 降低了 BK 聚瘤病毒感染的规律

Baptiste Demey¹, Aurélien Aubry¹, Virginie Morel¹

¹Virology Department, Amiens University Hospital Center, Amiens, 80000, France; AGIR Laboratory UR 4294, Picardie-Jules Verne University, Amiens, 80000, France.

Antiviral research

|November 27, 2025

概括

在移植受体中,JC多列马病毒 (JCPyV) 感染抑制了BK多列马病毒 (BKPyV) 复制,可能是通过病毒微RNA. JCPyV微RNA jcv-miR-J1-5p 特别减少了 BKPyV TAg 的表达,限制了 BKPyV 的感染力.

科学领域:

病毒学病毒学
免疫学免疫学免疫学
分子生物学分子生物学

背景情况:

BK多种类型病毒 (BKPyV) 和JC多种类型病毒 (JCPyV) 是密切相关的病毒,它们会导致持续性感染.
在移植接受者中,JCPyV复制可以预防BKPyV相关的病理.
病毒微RNAs (miRNAs) 在BKPyV和JCPyV之间是同源的,这表明在物种间的竞争中发挥了作用.

研究的目的:

研究JCPyV在移植受体中抑制BKPyV复制的机制.
确定病毒miRNAs在BKPyV和JCPyV之间观察到的竞争中的作用.

主要方法:

对39名脏移植接受者的病例控制研究,以评估尿液jcv-miR-J1-5p水平与BKPyVDNAemia之间的关联.
在实验室模型中,使用人类脏近端管状上皮细胞来研究JCPyV对BKPyV生长的影响.
设计一个原型的JCPyV菌株来灭miRNA成熟,以确认miRNA在JCPyV的抑制作用中的作用.

主要成果:

早期发现尿路jcv-miR-J1-5p显著降低了BKPyVDNAemia的风险 (OR=0.00 [0.00-0.65],p=0.012).
在体外,JCPyV感染减少了BKPyV的生长,而没有显著的JCPyV蛋白表达.
特定于JCPyV的miRNA jcv-miR-J1-5p降低了BKPyV TAg mRNA的表达,类似于BKPyV编码的miRNAs.

结论:

特定于JCPyV的miRNA jcv-miR-J1-5p限制了BKPyV的感染力和TAg的早期表达.
这种由miRNA介导的JCPyV抑制有助于在BCPyV和JCPyV感染之间观察到的体内竞争.
病毒miRNA交叉活性是解释JCPyV对移植受体BKPyV病理的保护作用的关键机制.

关键词:

在BK多重病毒中,BK多重病毒.在JC的多重病毒中,多重病毒有很多种.腎臟移植腎臟移植超级感染排除的排除这是一个微型RNA.

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