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Updated: Jun 25, 2026

Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris

Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris

Published on: February 25, 2010

在Pichia pastoris中系统优化热稳定的zearalenone水解酶ZENM的高水平分泌表达

Shida Zhao¹, Qin Zhou², Yuzhuo Wu³

¹College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, 471000, China; Institute of Veterinary Immunology & Engineering, Jiangsu Academy of Agricultural Sciences, Jiangsu, China.

Protein expression and purification

|November 27, 2025

概括

谷物中的泽拉伦 (ZEN) 污染威胁着牲畜. 在Pichia pastoris中表达的ZEN酸ZENM具有很高的热稳定性和活性,为工业用途提供具有成本效益的排毒解决方案.

科学领域:

生物技术是生物技术.
酶工程是什么? 酶工程是什么?
食品安全食品安全

背景情况:

泽拉 (ZEN) 是谷物谷物中普遍存在的真菌毒素,对牲畜健康构成风险.
酸酸对于有效和经济的酸排毒至关重要.
酸ZEN 酸ZENM 具有卓越的热稳定性,使其成为工业应用的主要候选者.

研究的目的:

在Pichia pastoris中异质地表达ZEN酸ZENM.
优化ZENM生产,以实现潜在的工业规模应用.
描述ZENM的酶性质并提高产量.

主要方法:

在Pichia pastoris系统中ZENM的异质表达.
使用信号和基因剂量的表达的优化.
与分子伴侣 (PDI,SEC12) 共同表达以提高产量.
酶活性测定和动力参数的确定.

主要成果:

在pH 8.0和60°C时,ZENM表现出最佳活性,在50-60°C之间具有90%以上的相对活性.
确定了运动参数 (K_m,app,V_max) 的时间.
通过信号优化,增加基因剂量和与伴侣共同表达,ZENM产量显著增加.
在震动瓶培养中,ZENM产量增加了10.79倍.

结论:

优化的Pichia pastoris表达系统为高产量的ZENM生产提供了一个强大的平台.
增强ZENM生产对于开发具有成本效益的真菌毒素排毒策略至关重要.
这项研究为ZENM在料和食品安全方面的工业应用奠定了基础.

关键词:

基因剂量基因剂量分子伴侣的共同表达.这就是Pichia pastoris.信号的优化信号的优化泽拉伦没有一个.

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