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Updated: Jan 10, 2026

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain
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Published on: December 12, 2017

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一个强大的协议,用于重新折叠TolC和其他外部膜组件的三方排泄从包含体的排泄

Andrew Daufel1, Andrés Cordova1, S Jimmy Budiardjo2

  • 1Department of Molecular Biosciences, University of Kansas, Lawrence, KS, United States.

Methods in enzymology
|November 27, 2025
PubMed
概括

抗生素耐药性是一个越来越令人担忧的问题. 一个新的协议通过优化重新折叠条件,提高了三聚体TolC的产量,这是Gram-负细菌排泄中的关键蛋白质.

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