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Updated: Jan 10, 2026

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通过交叉链接稳定多药物流量载体MdfA和抗体碎片组合,用于Cryo-EM单颗粒分析

Satomi Inaba-Inoue1, Toshio Moriya1, Norimichi Nomura2

  • 1Structual Biology Research Center, Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK), Tsukuba, Ibaraki, Japan.

Methods in enzymology
|November 27, 2025
PubMed
概括

抗体碎片稳定小膜蛋白,用于结构分析. 一种新的交叉链接方法改善了冷EM单颗粒分析,即使是对弱结合抗体.

关键词:
抗体碎片的一部分.交叉链接是指交叉链接.低温电磁单颗粒分析DHA家族的DHA家族是什么意思在MFS中,运输商是MFS运输商.多种药物排放输送器稳定化 稳定化 稳定化

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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