融合流介导的介酶体力驱动胚胎前肠收缩和分裂
Rui Yan1, Ludwig A Hoffmann2, Panagiotis Oikonomou3
1Department of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA, USA.
Nature communications
|November 27, 2025
概括
介质细胞通过压力驱动胚胎发育期间的上皮管分裂. 这一过程对于形成各种动物结构至关重要,可以受到像Sonic hedgehog (SHH) 这样的信号的影响.
科学领域:
- 发育生物学是发展生物学.
- 生物物理学的生物物理.
- 形态发生 形态发生 形态发生
背景情况:
- 表皮板转化为3D结构是动物形式多样性的关键.
- 已知上皮质折叠机制,但管分裂仍然不太了解.
- 表皮分裂涉及极端的折叠和拓过渡.
研究的目的:
- 研究表皮管分裂的生物力学基础.
- 使用四足动物胚胎发生过程中保存的气管-食道分离 (TES) 事件作为模型.
- 确定控制上皮质分裂的力量和信号通路.
主要方法:
- 研究了胚胎发育中的气管-食道分离 (TES).
- 分析了周围介质和细胞迁移的作用.
- 研究了索尼克刺 (SHH) 信号的功能.
- 使用外部压力应用来挽救缺陷.
主要成果:
- 确定了来自周围介质体的保存压力,导致上皮质收缩和分裂.
- 中细胞向上皮质的融合流调解了这种力.
- SHH信号吸引介质细胞到上皮层.
- 介质酶的去除,细胞迁移的抑制或SHH信号的丧失会破坏TES.
- 外部压力可以挽救TES的废除.
结论:
- 揭示了上皮管分裂的生物力学基础.
- 由SHH指导的介酶体压力对TES至关重要.
- 这些发现表明气管-食道先天缺陷的潜在介质原点.
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