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全基因组DNA甲基化和转录组集成分析揭示了A型大动脉剖析病原体的潜在标记物

Chao Chang¹, Meng Wang², Yunpeng Bai¹

¹Department of Cardiovascular Surgery, Tianjin Chest Hospital, Tianjin University, Tianjin, 300222, China.

Scientific reports

|November 27, 2025

概括

基因甲基化在A型大动脉剖析 (TAAD) 中发挥着作用. 研究人员确定了三个关键的甲基化基因,包括ZC3H12A,作为TAAD的潜在诊断标记物,ZC3H12A影响细胞行为.

科学领域:

血管生物学血管生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
基因组学就是基因组学.

背景情况:

甲型大动脉解剖 (TAAD) 是一种危及生命的血管疾病,死亡率高.
基因表观机制,特别是基因甲基化,TAAD病原体的基础仍然未被充分探索.

研究的目的:

通过整合基因甲基化和转录组数据来识别TAAD的新型候选诊断标记物.
调查已识别的甲基化基因在血管光滑肌细胞中的功能作用.

主要方法:

从TAAD和控制大动脉组织中整合甲基化和转录组测序数据.
生物信息学分析,包括差异分析,蛋白质-蛋白质相互作用网络和机器学习算法.
在体外实验中使用人类血管光滑肌细胞 (HVSMC) 来评估基因功能.

主要成果:

鉴定了239个不同的甲基化基因,主要涉及尼古丁胺核酸代谢.
发现了三个关键的甲基化基因 (ZC3H12A,IRAK2,CCL5) 作为潜在的TAAD标记物.
发现ZC3H12A促进了HVSMC的扩散和迁移,同时抑制了表型切换,其表达与甲基化水平负相关.

结论:

ZC3H12A,IRAK2和CCL5是A型大动脉剖析的潜在诊断生物标志物.
ZC3H12A可能通过影响血管光滑肌肉细胞行为,在TAAD进展中发挥重要作用.

关键词:

在 ZC3H12A 中.生物信息学分析甲基化序列的测序方法转录基因组序列的测序一种类型的甲动脉剖析.

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