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Updated: Jan 10, 2026

Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells

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Published on: December 17, 2015

周周病原体通过表面附着蛋白酶从人类的氨酸-氨酸系统降解血管新素I

Irena Waligórska¹, Krzysztof M Żak², Natalia Mikrut³

¹Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland. irena.waligorska@gmail.com.

Scientific reports

|November 28, 2025

概括

口腔病原体 Porphyromonas gingivalis 和 Tannerella forsythia 使用 PepO 蛋白酶调节氨酸 - 血管新生素系统 (RAS). 这些细菌引导RAS产生Ang 1-7,影响血压调节.

科学领域:

微生物学微生物学
生物化学生物化学
生理学生理学生理学

背景情况:

氨酸 - 血管素系统 (RAS) 通过血管素 (Ang) 调节血压.
口腔病原体 Porphyromonas gingivalis (Pg) 和 Tannerella forsythia (Tf) 都与系统性疾病有关.
口腔病原体与宿主系统 (如RAS) 相互作用的具体机制尚未完全理解.

研究的目的:

研究口腔病原体Pg和Tf在调节氨酸-血管新生素系统 (RAS) 中的作用.
为了确定负责血管素水解的特定细菌蛋白酶.
描述这些蛋白酶的酶性质和结构特征.

主要方法:

使用合成基质进行酶活性测定以表征PepO蛋白酶.
确定TfPepO的晶体结构,以阐明其催化机制.
免疫测试检测PepO在外膜囊泡中的释放.
盖勒里亚梅隆氏菌感染模型用于评估细菌毒性.

主要成果:

Pg 和 Tf 具有 PgPepO 和 TfPepO 的内酶,它们能化 Angiotensin I (Ang I).
由于其广泛的催化裂,TfPepO具有独特的基质水解特性.
佩波蛋白酶通过外膜囊泡从细菌细胞表面释放出来.
在Galleria mellonella模型中,PepO删除显著降低了Tf的毒性.

结论:

来自Pg和Tf的PepO蛋白酶是Ang I的水解中的关键介质.
这些细菌蛋白酶可以通过指导血管素的产生来调节宁-血管素系统 (RAS).
这些发现揭示了口腔病原体与宿主生理系统之间相互作用的新机制.

关键词:

葡萄牙牙牙病 Porphyromonas gingivalis 这种虫子是这种植物是Tannerella forsythia.牙周病是指牙周病的发生.蛋白质酶可以保护雷宁 - ангиотензин 系统

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