相关实验视频

Updated: Jan 10, 2026

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Published on: March 27, 2020

[circ_EPHB4与YTHDF3协同作用,通过m6的A-依赖稳定Wnt3促进质瘤的进展

Chen Jin¹, Jingping Liu¹, Bo Liu¹

¹Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha 410008, China.

Nan fang yi ke da xue xue bao = Journal of Southern Medical University

|November 28, 2025

概括

循环RNAcirc_EPHB4和YTHDF3蛋白质通过共同准Wnt3信号通路来促进质瘤的进展. 这种相互作用增强了细胞迁移,入侵和瘤性,为质瘤提供了潜在的新治疗策略.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

质瘤是一种原发性脑瘤,预后不佳.
循环RNAs (circRNAs) 正在成为各种癌症的关键调节者,包括质瘤.
质瘤中circ_EPHB4的特定作用和机制在很大程度上仍未被探索.

研究的目的:

为了研究circ_EPHB4在质瘤中的致癌作用.
阐明circ_EPHB4在质瘤进展中的功能背后的分子机制.
探索circ_EPHB4作为质瘤治疗点的潜力.

主要方法:

微阵列分析以确定质瘤组织中差异表达的circRNAs.
在体外测试 (伤愈合,Transwell入侵) 和体内裸体小鼠模型,以评估circ_EPHB4对细胞迁移,入侵和瘤性的影响.
RNA免疫沉 (RIP),RNA稳定性测定和基因过度表达/沉默技术,以研究circ_EPHB4和YTHDF3对Wnt3表达的协同调节作用.

主要成果:

在质瘤组织和细胞中,Circ_EPHB4显著过度表达.
过度表达circ_EPHB4促进了质瘤细胞的迁移,入侵,上皮层-介质细胞过渡 (EMT),以及体内瘤的生长.
Circ_EPHB4上调Wnt3表达,而YTHDF3以m6A依赖的方式稳定Wnt3mRNA,联合淘汰显著降低Wnt3表达.

结论:

通过准Wnt3信号通路,Circ_EPHB4和YTHDF3合作促进质瘤的进展.
圆环EPHB4/YTHDF3/Wnt3轴代表了潜在的新型治疗策略,用于质瘤治疗.

关键词:

没有Wnt3 这就是YTHDF3的原因.电路_EPHB4 电路质瘤质瘤是一种 m 6 A A 6 年的时间.

更多相关视频

Quantitative Immunohistochemistry of the Cellular Microenvironment in Patient Glioblastoma Resections

Quantitative Immunohistochemistry of the Cellular Microenvironment in Patient Glioblastoma Resections

Published on: July 31, 2017

Induction and Analysis of Epithelial to Mesenchymal Transition

Induction and Analysis of Epithelial to Mesenchymal Transition

Published on: August 27, 2013

相关概念视频

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...

PI3K/mTOR/AKT Signaling Pathway

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...

Tumor Progression

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...

Epigenetic Regulation

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...

Epigenetic Regulation

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.