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MdDREB2A通过抑制MdPR基因表达来负面调节果对Alternaria alternata的抵抗力

Chana Bao^1,2, Gege Qin², Chen Liu²

¹Northwest A&F University ShenZhen Research Institute, Shenzhen, Guangdong, China.

Plant, cell & environment

|November 28, 2025

概括

一个关键的转录因子MdDREB2A通过抑制与病变发生相关的 (PR) 基因来抑制果对叶子斑病的免疫力. 了解这种相互作用为开发抗病果品种提供了新的策略.

科学领域:

植物病理学植物病理学
分子生物学分子生物学
植物与微生物的相互作用

背景情况:

叶子斑病,由Alternaria alternata f. sp. 引起的马里,显著影响果产量.
与病原发生相关的 (PR) 基因对植物免疫至关重要,但它们的调节机制尚未完全理解.

研究的目的:

研究一种转录因子MdDREB2A在果对Alternaria alternata的防御中的作用.
阐明果免疫中涉及MdDREB2A和致病相关 (PR) 基因的调控网络.

主要方法:

DAP-seq分析以确定MdDREB2A的直接目标.
ChIP-qPCR,EMSA和双露西法酶测定验证了基因调节.
对MdDREB2A过度表达和淘汰植物对疾病易感性的分析.

主要成果:

MdDREB2A通过抑制MdPR10基因表达来负面调节果对A. alternata的耐药性.
过度表达MdDREB2A增加了敏感性,而敲击则增强了抵抗力.
MdDREB2A直接结合并抑制三种具有抗真菌活性的MdPR10基因的转录.

结论:

MdDREB2A充当了对果中的A. alternata防御的负调节者.
MdDREB2A对MdPR10基因的抑制降低了抗真菌耐药性.
这一发现为开发抗叶斑病的果品种提供了一个新的目标.

关键词:

A. 替代性的交换机在MdDREB2A中.在MdPR10中使用.果果果果是什么意思疾病耐药性疾病耐药性

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