柏柏林对患有性结肠炎的小鼠肠道微生态的调节作用
Xinyi Xu1, Bin Zhao2, Pingyu Liu3
1Graduate School of Shanghai Medical College, Fudan University, Shanghai, China.
Frontiers in microbiology
|November 28, 2025
概括
柏柏林 (BBR) 通过改善肠道微生物群和代谢物概况,有效治疗小鼠的性结肠炎 (UC). 阻止PDGFA受体逆转了BBR的益处,恶化了炎症和肠道屏障损伤.
科学领域:
- 胃肠病学 胃肠病学
- 药理学 药理学是指药理学的学科.
- 微生物组研究 微生物组研究
背景情况:
- 目前的性结肠炎 (UC) 治疗有诸如副作用,高成本和低于最佳反应等局限性.
- 柏柏林 (BBR) 是一种传统中医药成分,具有抗炎和肠道微生物群调节的特性,具有低毒性.
- BBR提出了一种潜在的新型治疗策略,用于UC.
研究的目的:
- 在小鼠模型中研究柏柏林 (BBR) 对性结肠炎 (UC) 的治疗作用.
- 阐明涉及肠道微生物群,代谢物和宿主基因相互作用的潜在机制.
- 探索PDGFA信号通路在BBR治疗作用中的作用.
主要方法:
- 建立了一个由硫酸 (DSS) 诱导的UC的小鼠模型.
- 进行剂量和时间依赖的查以优化BBR干预.
- 评估疾病活性,结肠组织学,血清细胞因子,粘膜屏障完整性 (ZO-1,Occludin),肠道微生物群组成 (16S rRNA测序) 和肠道代谢学.
- 利用门德尔的随机化 (MR) 分析来确定基因,代谢物和细菌之间的因果关系.
- 通过涉及PDGFA受体抑制的功能实验验证实发现.
主要成果:
- 柏柏林显著缓解了UC症状,减少了结肠炎症,并恢复了小鼠的粘膜屏障功能.
- BBR治疗调节了肠道微生物群的组成,增加了有益细菌的相对丰富程度,如*Bacteroides*和*Alistipes*.
- 代谢分析显示了肠道代谢物的变化,MR分析确定了涉及PDGFA,石灰酸硫酸盐和*Alistipes*的因果链.
- 抑制PDGFA受体逆转了BBR的治疗作用,加剧了炎症和屏障损伤.
结论:
- 柏柏林通过调节肠道微生物群和代谢物概况来改善小鼠的UC症状.
- 在PDGFA,石灰酸硫酸盐和*Alistipes*丰度之间确立了显著的因果关系.
- 准PDGFA受体通路对于理解和潜在地提高BBR在UC治疗疗效至关重要.
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