在玉米生物质中工程积累原始甲酸盐,以提高生物制造
Yang Tian1,2, Bumkyu Kim3,4,5, Irem Pamukçu2
1Joint BioEnergy Institute, Emeryville, California 94608, United States.
概括
工程玉米现在在生物质中产生了高水平的原始甲酸盐 (DHBA). 这一进步增强了玉米炉的性能.
科学领域:
- 生物技术和生物工程 生物技术和生物工程
- 植物分子生物学 植物分子生物学
- 可持续化学 可持续化学
背景情况:
- 玉米是生物燃料和生物制品的有价值的纤维素生物质资源.
- 植物内共产品的积累可以提高生物质价值并支持生物经济.
研究的目的:
- 为了在玉米的生物质中过度生产原始甲基酸盐 (DHBA),对玉米进行工程.
- 评估DHBA积累对生物质组成和下游加工的影响.
主要方法:
- 转基因玉米系是使用细菌脱水石基马酸脱水酶 (QsuB) 基因创建的.
- 分析了DHBA积累,素组成和可提取的化合物.
- 生物质水解物的生物升级使用工程细菌进行.
主要成果:
- 转基因玉米在叶子和茎生物质中以糖化物形式积累了高达2.9%的DHBA (干重量).
- DHBA的积累改变了素的组成,增加了氨酸/氨酸的比率和p-coumarate的丰富度.
- 来自QsuB玉米的水解剂的生物升级产生了375高的2-pyrone-4,6-dicarboxylate标位.
结论:
- 对玉米进行基因工程以过度生产DHBA是可行的,并提高生物质价值.
- 富含DHBA的玉米便于改善生物产品的制造.
- 该战略为从农业废物中可持续生产生物燃料和化学品提供了一条途径.
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