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The Replisome

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...

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Updated: Jan 10, 2026

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Published on: April 12, 2019

可编程循环sDNA生产和可扩展DNA原始组装的和消化策略.

Jingyi Ye^1,2, Ao Liu², Hui Lv³

¹School of Biomedical Sciences and Engineering, South China University of Technology, Guangzhou International Campus, Guangzhou 511442, China.

|November 28, 2025

概括

此摘要是机器生成的。

这项研究引入了一种新方法,可以为DNA原形创建长长的DNA支架,克服尺寸限制. 改进的技术使得更大,更稳定的纳米结构能够用于先进的应用.

关键词:

原子力显微镜 (AFM) 的使用圆形单链DNA (cssDNA) 是一种单链DNA.DNA分子动力学DNA分子动力学基因原始的DNA原始化一个组装组件.

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Folding and Characterization of a Bio-responsive Robot from DNA Origami

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Published on: December 3, 2015

Designing a Bio-responsive Robot from DNA Origami

Designing a Bio-responsive Robot from DNA Origami

Published on: July 8, 2013

相关实验视频

Last Updated: Jan 10, 2026

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Published on: April 12, 2019

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Published on: December 3, 2015

Designing a Bio-responsive Robot from DNA Origami

Designing a Bio-responsive Robot from DNA Origami

Published on: July 8, 2013

科学领域:

纳米技术纳米技术
合成生物学合成生物学
生物化学生化学

背景情况:

DNA原始结构允许精确的纳米结构组装用于各种应用.
可扩展性受到可用的单链DNA (ssDNA) 支架的长度的限制.

研究的目的:

开发一种用于生成长圆形ssDNA (cssDNA) 可定制长度的支架的方法.
优化大规模的DNA原木组装,以提高效率和稳定性.

主要方法:

一种使用Cas9尼克酶 (Cas9n) 和T7外核酶 (T7 Exo) 来从等离子体DNA中产生cssDNA的"和消化"策略.
在单折叠中优化变质温度,回火和主与支架的比率.
使用原子力显微镜 (AFM) 和分子动力学模拟进行表征.

主要成果:

成功生成了高纯度的7k-nt和15k-ntcssDNA支架,其序列依赖性最小.
实现了大规模DNA原始结构 (147 × 107 nm) 的单折叠,与传统设计相比,表面积翻了一番.
证明了更高的产量,更少的结构缺陷,在一组装与两步方法相比,提高了机械稳定性.

结论:

"和消化"策略为生成长cssDNA支架提供了一个强大的方法.
优化的一组装克服了DNA原形可扩展性和可编程性的关键障碍.
这种方法促进了大规模纳米电子,数据存储和治疗方面的应用.