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可编程循环sDNA生产和可扩展DNA原始组装的和消化策略

Jingyi Ye^1,2, Ao Liu², Hui Lv³

¹School of Biomedical Sciences and Engineering, South China University of Technology, Guangzhou International Campus, Guangzhou 511442, China.

|November 28, 2025

概括

这项研究引入了一种新方法,可以为DNA原形创建长长的DNA支架,克服尺寸限制. 改进的技术使得更大,更稳定的纳米结构能够用于先进的应用.

科学领域:

纳米技术纳米技术
合成生物学合成生物学
生物化学生化学

背景情况:

DNA原始结构允许精确的纳米结构组装用于各种应用.
可扩展性受到可用的单链DNA (ssDNA) 支架的长度的限制.

研究的目的:

开发一种用于生成长圆形ssDNA (cssDNA) 可定制长度的支架的方法.
优化大规模的DNA原木组装,以提高效率和稳定性.

主要方法:

一种使用Cas9尼克酶 (Cas9n) 和T7外核酶 (T7 Exo) 来从等离子体DNA中产生cssDNA的"和消化"策略.
在单折叠中优化变质温度,回火和主与支架的比率.
使用原子力显微镜 (AFM) 和分子动力学模拟进行表征.

主要成果:

成功生成了高纯度的7k-nt和15k-ntcssDNA支架,其序列依赖性最小.
实现了大规模DNA原始结构 (147 × 107 nm) 的单折叠,与传统设计相比,表面积翻了一番.
证明了更高的产量,更少的结构缺陷,在一组装与两步方法相比,提高了机械稳定性.

结论:

"和消化"策略为生成长cssDNA支架提供了一个强大的方法.
优化的一组装克服了DNA原形可扩展性和可编程性的关键障碍.
这种方法促进了大规模纳米电子,数据存储和治疗方面的应用.

关键词:

原子力显微镜 (AFM) 的使用圆形单链DNA (cssDNA) 是一种单链DNA.DNA分子动力学DNA分子动力学基因原始的DNA原始化一个组装组件.

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