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从野生印度大象 (Elephas maximus indicus) 获取的 Pseudodiscus collinsi 的分子鉴定,基于 ITS-2 标准

A Anaswara¹, S Arun², R Geethu¹

¹Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Pookode, Kerala Veterinary and Animal Sciences University, Wayanad, Kerala 673 576, India.

|November 28, 2025

概括

两胞,一种巧合性疾病,影响许多动物. 这项研究使用形态学和DNA识别了印度大象中的Pseudodiscus collinsi,证实它是一种独特的物种.

科学领域:

兽医寄生虫学兽医寄生虫学
分子遗传学分子遗传学
野生动物健康野生动物健康

背景情况:

由Paramphistomoidea flukes引起的两虫病是一种全球重要的寄生虫疾病,影响着不同的宿主.
众所周知,该物种*Pseudodiscus collinsi*感染马匹和大象,主要是在结肠中.
两菌病的患病率和影响往往被低估,这凸显了需要详细的物种识别和表征的需要.

研究的目的:

为了在形态和分子上识别从野生印度大象中恢复的巧合标本.
为了建立第一个分子标志物为*Pseudodiscus collinsi*.
为了澄清感染大象的P. collinsi的遗传位置.

主要方法:

使用乙卡明染色,以口腔袋和丸为重点,对flukes的形态识别.
通过聚合酶链反应 (PCR) 放大内部转录间隔器2 (ITS-2) 区域的分子鉴定.
使用最大概率 (ML) 方法与基穆拉2参数模型进行遗传学分析.

主要成果:

从印度大象身上回收的被形态识别为*Pseudodiscus collinsi*.
来自大象的P. collinsi的ITS-2区域成功地被放大和测序.
遗传学分析证实,来自大象的P. collinsi是一种独立的物种,与其他两动物分开.

结论:

这项研究提供了来自大象的* Pseudodiscus collinsi* 的第一个分子数据.
这些发现支持P. collinsi感染大象的独特物种地位.
这项研究有助于更好地了解两体多样性和宿主特异性.

关键词:

两形体是两形体.在基因方面,特里马托德是基因上的特里马托德.在 ITS-2 系统中使用.印度大象印度大象伪科林斯 (Pseudodiscus collinsi) 是一种类型的植物.

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