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通过Keap1/Nrf2通路,化 pea (Pisum sativum L.) 的抗氧化应激作用

Qian Li^1,2, Yonggan Yuan^1,2, Mingwu Qiao^1,2

¹Henan Engineering Technology Research Center of Food Processing and Circulation Safety Control, College of Food Science and Technology, Henan Agricultural University, Zhengzhou, China.

Journal of the science of food and agriculture

|November 28, 2025

概括

这项研究准备了化豆 (CPP),并发现它们具有增强的抗氧化活性. 通过调节Keap1/Nrf2/HO-1通路,CPP有效地减少了胰岛素抵抗细胞模型中的氧化应激.

科学领域:

生物化学和分子生物学
营养科学营养科学
材料科学材料科学材料科学

背景情况:

三价 (Cr(III)) 对于葡萄糖和脂质代谢至关重要.
豆类 (PP) 可以化Cr(III),但其机制和活性尚未完全理解.
了解Cr (III) - 相互作用对于代谢健康研究至关重要.

研究的目的:

为了制备基化豆 (CPP).
描述 CPP 的结构.
为了研究CPP的抗氧化应激效应.

主要方法:

用于CPP制备的生物丰富方法.
使用UV-Vis,光谱学,FTIR,SEM和HPLC-MS进行结构性表征.
在体外抗氧化剂测试 (DPPH,基清除,铁还原) 和建立胰岛素抵抗的HepG2细胞模型.

主要成果:

证实了离子通过炭基,碳基和氨基配体有效地与PP结合.
与PP相比,CPP的抗氧化活性显著更高.
化部位涉及C=O,N-H和O-H组.

结论:

CPP调节了Keap1/Nrf2/HO-1通路,促进了Nrf2的核转移.
通过CPP激活血红素氧酶-1 (HO-1),有助于产生抗氧化作用.
在一种抗胰岛素的HepG2细胞模型中,CPP有效降低了氧化应激.

关键词:

在HepG2细胞中.抗氧化剂活性抗氧化剂活性生物丰富是一种生物丰富.是的元素之一.豆的基化酸.氧化应激是一种氧化应激.

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