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适应性实验室进化Saccharomyces cerevisiae CEN.PK 113-7D以提高乙醇耐受性

Fatemeh Sheikhi^1,2, Mahsa Babaei³, Khosrow Rostami¹

¹Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), Tehran 331319-3685, Iran.

FEMS yeast research

|November 28, 2025

概括

适应性实验室进化增强了用于工业乙醇生产的Saccharomyces cerevisiae. 进化酵母菌株显著改善了生长率和乙醇产量,特定的基因突变被确定为耐受性的关键驱动因素.

科学领域:

微生物学微生物学
生物技术是生物技术.
合成生物学合成生物学

背景情况:

在工业乙醇发酵中,Saccharomyces cerevisiae非常重要.
高度的乙醇,温度和透应激抑制了酵母发酵效率.

研究的目的:

通过适应性实验室进化改善Saccharomyces cerevisiae对高度乙醇的耐受性.
为了确定遗传突变赋予增强的乙醇耐受性和生产.

主要方法:

在144天的时间里,S. cerevisiae CEN.PK 113-7D的适应性实验室进化,以增加乙醇度.
全基因组测序以确定进化菌株中的突变.
通过将它们重新引入非进化酵母中来验证已识别的基因突变.

主要成果:

在进化菌株中观察到最大特异性生长率 (μmax) 和特异性葡萄糖吸收率的显著增加.
在11%的乙醇上,进化菌株产生了94.5g/L的乙醇,而非进化菌株则为78.5g/L.
在RKI1,CYC2,ANR2,RGA2,RGA1,LPX1和LRE1基因中验证的突变在9%的乙醇中增长1.7-5倍.

结论:

适应性实验室进化在产生具有增强乙醇耐受性和生产力的S. cerevisiae菌株方面是有效的.
特定的基因突变,特别是RKI1,CYC2,RGA2,RGA1和LPX1,对于提高发酵性能至关重要.
鉴定的突变为未来工业应用酵母的代谢工程提供了目标.

关键词:

这种植物是Saccharomyces cerevisiae.适应性实验室进化的演变.这就是CRISPR-Cas9的特征.乙醇的耐受性乙醇的耐受性反向工程是一种逆向工程.改进菌株,改善菌株.

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