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通过双重免疫诱导和病原体抑制,工程化埃利素蛋白增强了植物中的Phytophthora耐药性

Zhengpeng Li¹, Yong Pei², Hao Zhou²

¹Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection/Jiangsu Key Laboratory for Eco-Agricultural Biotechnology Around Hongze Lake, Huaiyin Normal University, Huai'an, 223300, China.

|November 28, 2025

概括

改造的SOJ5^V84F蛋白质通过阻断固醇信号传递和激活植物免疫力来抑制Phytophthora的生长. 这种双重行动战略为农作物提供了加强的生物控制,以对抗破坏性的虫病原体.

科学领域:

植物病理学植物病理学
分子生物学分子生物学
生物技术是生物技术.

背景情况:

植物病原体是农业的主要威胁,因为它们无法合成固醇,依赖宿主植物.
固醇辅性对Phytophthora物种构成弱点,为疾病控制提供了潜在的目标.

研究的目的:

为了设计一种双重功能的乙素蛋白,SOJ5^V84F,用于增强Phytophthora病原体的生物控制.
研究SOJ5^V84F在抑制病原体生长和激活植物防御方面的机制.

主要方法:

在Phytophthora sojae elicitin SOJ5的部位定向突变生成,以创建V84F变种.
评估SOJ5^V84F与固醇感应受体激酶SSRK1.1的相互作用.
评估SOJ5^V84F对Phytophthora生长,固醇信号通路和植物免疫反应 (ROS爆发) 的影响.
温室试验测试SOJ5^V84F对各种作物的Phytophthora物种的有效性.

主要成果:

V84F突变取消了固醇结合,但保持了SSRK1相互作用,使SSRK1-介导信号的主导负抑制成为可能.
SOJ5^V84F显著抑制了P. sojae以SSRK1依赖的方式生长.
SOJ5^V84F有效地引起了大豆,胡,番茄和土豆植物中的反应性氧物种 (ROS) 爆发.
与野生类型的SOJ5相比,使用SOJ5^V84F进行预处理可以提供对Phytophthora病原体的更强的保护.

结论:

设计的SOJ5^V84F通过直接抑制病原体生长和激活宿主免疫力,提供了一种新的双机制生物控制策略.
这种基于蛋白质的方法显示出在农业中控制由醇-辅性菌体引起的疾病的巨大潜力.

关键词:

植物植物群 (Phytophthora) 是一种植物群.埃尔西丁是一种素.由模式触发的免疫力这是一种固醇 (Sterol).

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