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相关概念视频

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
11.7K

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相关实验视频

Updated: Jan 10, 2026

Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection
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来自单细胞RNA-seq的B型肝炎病毒转录丰度和基因组段分布量化的协议.

Lina Liu1, Liyu Chen2, Lingyun Zhou2

  • 1Center of Infectious Diseases, West China Hospital of Sichuan University, Chengdu, China; Laboratory for Pathogen Research, West China Hospital, Sichuan University, Chengdu, China.

STAR protocols
|November 28, 2025
PubMed
概括

这项研究引入了一种新的协议,用于分析乙型肝炎病毒 (HBV) 感染肝脏组织的单细胞. 它允许详细检查病毒基因表达和宿主相互作用在细胞水平.

关键词:
生物信息学是一种生物信息学.细胞生物学 细胞生物学基因表达 基因表达 基因表达卫生科学 卫生科学分子生物学分子生物学序列分析是指进行序列分析.一个单细胞的单细胞.

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A Protocol for Analyzing Hepatitis C Virus Replication
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科学领域:

  • 肝病学 肝病学是一种肝病学.
  • 病毒学 病毒学
  • 分子生物学分子生物学

背景情况:

  • 乙型肝炎病毒 (HBV) 感染是慢性肝病和肝细胞癌的主要原因.
  • 在细胞水平上了解HBV与宿主之间的相互作用对于开发有效疗法至关重要.

研究的目的:

  • 为单细胞分析HBV感染肝脏组织提出一个全面的方案.
  • 为了能够对单细胞中的HBV转录丰度和全基因组读取分布进行定量评估.

主要方法:

  • 组织解离和净化来自HBV感染肝脏组织的细胞.
  • 单细胞RNA测序 (scRNA-seq) 库的准备,测序和数据处理.
  • 分析HBV表达模式和宿主细胞内的病毒基因组分布.

主要成果:

  • 该协议产生了量化每细胞HBV转录丰度数据.
  • 阅读分布的全基因组图为病毒整合和表达提供了洞察力.
  • 通过单细胞分辨率实现了病毒表达模式和HBV与宿主相互作用的详细分析.

结论:

  • 该协议为剖析单细胞水平上HBV感染的复杂性提供了一个强大的工具.
  • 它有助于更深入地了解HBV感染肝脏中的病毒病原和宿主反应.
  • 该方法支持识别新的治疗点和管理HBV相关肝病的策略.