一种方法,通过LC/ESI-MS/MS同时确定指甲切片中的trioidothyronine和thyroxine
Kenjiro Shibata1, Sakurako Tanaka1, Shuhei Baba2
1Faculty of Pharmaceutical Sciences, Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku, Tokyo 125-8585, Japan.
概括
切割的指甲可以随着时间的推移测量甲状腺激素trioidothyronine (T3) 和thyroxine (T4). 一种新的LC/ESI-MS/MS方法准确量化了指甲中的这些激素,显示了临床应用的潜力.
科学领域:
- 内分泌学和新陈代谢学
- 分析化学 分析化学
- 生物标志物发现发现
背景情况:
- 切断指甲是适合评估长期激素状况的.
- 甲状腺激素 (T3和T4) 在指甲中的临床意义尚不清楚.
- 需要准确的量化方法来评估甲状腺激素在指甲样本中的水平.
研究的目的:
- 开发和验证一个方法量化三甲氨酸 (T3) 和甲状腺素 (T4) 在指的剪切.
- 评估指甲激素水平用于评估甲状腺状况的临床适用性.
主要方法:
- 开发了一种液体色谱/电子喷雾电离-并联质谱法 (LC/ESI-MS/MS) 方法.
- 用50%的乙醇提取了指甲T3和T4,并使用Strata-X弹进行净化.
- C6 - T4被用作内部标准;方法验证包括精度和恢复评估.
主要成果:
- 该LC/ESI-MS/MS方法显示了高精度 (RSD ≤4.5%) 和准确性 (98.3-103.3%的恢复).
- 量化下限为T3和T4的0.20 ng/g.
- 甲状腺素受试者 (n=40) 的指甲T3 (0.74 ± 0.26 ng/g) 和T4 (0.90 ± 0.27 ng/g) 在相似水平.
- 患有格雷夫斯病的患者指甲T3和T4水平显著提高.
- 患有哈西莫托甲状腺炎的患者表现出较低指甲T3和T4水平的趋势.
结论:
- 开发的LC/ESI-MS/MS方法可靠地量化指甲切片中的T3和T4.
- 指甲T3和T4水平与甲状腺疾病状态相关 (格雷夫斯病,哈希莫托甲状腺炎).
- 这种方法为评估中长期甲状腺激素产量和临床状态提供了有价值的工具.
关键词:
切割指甲 切割指甲 切割指甲格雷夫斯病是什么?甲状腺功能低下症 甲状腺功能低下症在 LC/ESI-MS/MS 中.它与蛋白质结合.甲状腺素 甲状腺素三氨基氨酸 (Triiodothyronine) 是一种氨酸.更多相关视频
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