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在热友细胞酶2-EPIMERASE增强低温乳糖生物合成中平衡α6/α6桶动力稳定性和循环灵活性

Erum Bux¹, Magezi Joshua¹, Hero Nmeri Godspower¹

¹The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, Jiangsu, China..

International journal of biological macromolecules

|November 28, 2025

概括

改造的纤维素二胺酶 (CsCE) 在较低的温度下显示出改善的乳糖合成. 突变酶平衡酶的稳定性和灵活性增强了CsCE的活性,使成本有效的乳糖生产成为可能.

科学领域:

生物化学生物化学
酵素工程是什么意思酵素工程
生物催化剂是一种生物催化剂.

背景情况:

来自Caldicellulosiruptor saccharolyticus的纤维素-2-表皮酶 (CsCE) 在75°C时有效合成乳糖.
由于其高最佳温度要求,CsCE的工业应用受到阻碍.

研究的目的:

在较低的温度下增强CsCE活性和适应性,以改善乳糖的生产.
为了研究α6/α6桶和柔性环在CsCE的热性能中的作用.

主要方法:

针对CsCE的向性突变发生,重点关注在α6/α6桶附近的高RMSF残留物 (K223H,K223Y,G224N).
在柔性循环中引入普罗林到甘氨酸突变以增加局部灵活性 (P106G).
有益突变 (G224N和P106G) 的组合,以创建一个改进的CsCE变异.

主要成果:

单个突变K223H,K223Y和G224N显示出增强的活动.
这种P106G突变增加了局部灵活性.
组合的G224N和P106G变种在75°C,60°C和50°C分别实现了82%,68.13%和56.24%的乳糖转化.
在60°C和50°C观察到显著的生物转化,与野生类型的CsCE不同.

结论:

平衡酶稳定性和灵活性是改善CsCE在低于最佳温度的性能的一种可行的策略.
工程 CsCE 变体显示了在较低温度下更经济地生产乳糖的潜力.

关键词:

乳糖糖的生物合成环的灵活性环的灵活性 α6/α6 的桶.

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