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甲醇生物转化用于在酵母中生产可生物降解的塑料单体L-乳酸盐

Wei Yu^1,2,3, Chenyue Zhang⁴, Yunxia Li^1,2

¹Division of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.

Nature communications

|November 28, 2025

概括

研究人员利用酵母技术从甲醇中生产L-乳酸盐,一种可生物降解的塑料前体. 这种生物制造方法实现了高产量,为以石油为基础的塑料提供了可持续的替代品,并减少了二氧化碳排放.

科学领域:

生物技术和代谢工程生物技术和代谢工程
可持续的生物制造
聚合物科学聚合物科学

背景情况:

甲醇是生物制造的可行的原料.
从甲醇生产乳酸盐为减轻塑料污染提供了一个可持续的替代方案.
从甲醇生产乳酸盐的微生物工程面临挑战,原因是细胞生长的竞争途径.

研究的目的:

为了设计酵母Ogataea polymorpha从甲醇中有效合成L-乳酸盐.
优化辅助因子的可用性和细胞过程,以提高L-乳酸盐的生产.
评估二氧化碳衍生L-乳酸盐的商业可行性和环境影响.

主要方法:

在Ogataea polymorpha中重新连接代谢途径以进行L-乳酸盐合成.
工程基因表达和增强细胞活力.
修改辅助因子稳态,并利用线粒体的分离.
实施摇瓶和料批发发酵策略.
进行技术经济分析和生命周期评估.

主要成果:

在摇动瓶培养中达到2.5g/L的L-乳酸盐.
通过料批发发酵达到25.0 g/L L-乳酸盐的最大标位.
证明了从CO2中获得的甲醇中碳中和生产L-乳酸盐的潜力.
评估生物制造工艺的商业和环境可行性.

结论:

工程设计的Ogataea polymorpha可以有效地从甲醇中产生L-乳酸盐.
开发的生物制造工艺显示出可持续生产可生物降解塑料的前景.
这项研究为从二氧化碳中碳的多乳酸生产提供了基础.

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