转录基因洞察GSDME驱动的头部和部癌症中的火灭
Fengxiang Hao1,2, Danlei Qin1, Ran Li2
1Shanxi Medical University, Taiyuan, 030001, Shanxi Province, China.
Scientific reports
|November 29, 2025
概括
在头部和部平状细胞癌 (HNSCC) 中,与灭相关的基因 (PRG) 发生变化. 在HNSCC中高GSDME表达与降低的CK13/CK19相关,可能抑制瘤生长.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 头部和部状细胞癌 (HNSCC) 是一个重大的全球健康挑战.
- 编程细胞死亡途径的 Pyroptosis 和它相关的基因 (PRGs) 越来越多地被认为是癌症中的角色.
- 瘤免疫微环境显著影响HNSCC的进展和治疗反应.
研究的目的:
- 调查HNSCC.中与热致死相关基因 (PRG) 的表达模式和预后意义.
- 探索PRG,瘤免疫微环境和HNSCC发育之间的关系.
- 进行高GSDME表达的HNSCC的转录组分析,以确定潜在的分子机制.
主要方法:
- 利用TCGA-HNSCC和GSE65858数据集进行PRG的差异基因表达分析.
- 在基因和基因组的京都百科全书 (KEGG) 和基因本体学 (GO) 中对差异表达基因 (DEG) 进行了丰富分析.
- 使用CIBERSORTx评估免疫细胞透,并进行了转录基因测序 (Illumina NovaSeq 6000) 和免疫组织化学 (IHC) 测定GSDME过度表达的HNSCC.
主要成果:
- 在HNSCC中发生了17个PRG突变,TP53,CASP8和NLRP3显示出高突变频率.
- DEGs在与焦点粘附,细胞外基质和活性蛋白结合相关的途径中得到丰富.
- 高GSDME表达与CK13 (KRT13) 和CK19 (KRT19) 的表达显著下降,以及细胞粘附分子和酸通路中下调基因的丰富有关.
结论:
- 在HNSCC中,与灭相关的基因经常发生变化,可能会影响瘤免疫微环境.
- GSDME过度表达导致CK13和CK19的表达减少,这表明GSDME媒介抑制HNSCC生长的潜在机制.
- GSDME可能与特定的转录因子家族有关,包括ETS和Homeobox,这需要进一步调查.
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