血清中的元素和生物分子变化作为体内SPION作用的指标:使用TXRF,FTIR和拉曼光谱学的分析
Aleksandra Wilk1, Karolina W Lakomy1, Zuzanna Setkowicz2
1Faculty of Physics and Applied Computer Science, AGH University of Krakow, Al. Mickiewicza 30, 30-059, Krakow, Poland.
Scientific reports
|November 29, 2025
概括
超偏磁铁氧化物纳米粒子 (SPION) 改变血清成分,影响脂质,蛋白质和和等基本元素,表明潜在的毒性和需要在纳米医学中仔细监测.
科学领域:
- 纳米医学是一种纳米医学.
- 生物材料科学 生物材料科学
- 毒理学 毒理学 毒理学
背景情况:
- 超偏磁铁氧化物纳米粒子 (SPION) 显示出对诸如theranostics等医疗应用的前景.
- 由于对其系统生物相容性和生理影响的理解有限,因此SPION的临床使用受到阻碍.
- 确定SPION诱导的生物反应的生物标志物对于安全的临床实施至关重要.
研究的目的:
- 在施用SPION后,研究大鼠血清中的分子和元素变化.
- 为了确定纳米粒子诱导的生物反应的敏感生物标志物.
- 评估多模式光谱方法对监测SPION效应的有用性.
主要方法:
- 在Wistar大鼠中静脉注射PEGylated SPION.
- 在注射后2小时,24小时和7天收集血清样本.
- 使用富里埃变换红外 (FTIR) 光谱,拉曼光谱和总反射X射线光 (TXRF) 光谱进行多式分析.
主要成果:
- FTIR和拉曼光谱检测显示了血清生物分子的时间依赖性变化,包括降低脂质与蛋白质的比率,增加血红蛋白信号 (表明血液溶解),以及脂质和蛋白质结构的变化.
- TXRF光谱显示血清和,主要抗氧化剂的显著减少,铜和的动态波动,与一些性别依赖的趋势.
- 施用SPION诱导了协调的生理反应,包括红细胞损伤,氧化应激和元素平衡的破坏.
结论:
- 施用SPION会对血清成分产生显著的,时间依赖的全身性影响.
- 多模态光谱技术可以有效监测纳米粒子诱导的生物反应.
- 这些发现支持使用这些方法用于纳米毒理学和转化纳米医学研究.
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