在全身麻醉下的儿童中,MiR-18a-5p作为外科手术期间呼吸系统不良事件的危险因素
Lin Zhang1, Xinyuan Yu2, Jun Liu3
1The First Clinical Medical College, Heilongjiang University of Chinese Medicine, Harbin, 150400, China.
Perioperative medicine (London, England)
|November 29, 2025
概括
这项研究发现,miR-18a-5p是小儿麻醉中术前呼吸系统不良事件 (PRAE) 的重要预测和诊断生物标志物. 它的水平与炎症和患者因素相关,表明治疗潜力.
科学领域:
- 儿科麻醉学 儿科麻醉学
- 分子生物学分子生物学
- 生物标志物发现发现
背景情况:
- 术后呼吸系统不良事件 (PRAE) 在儿科麻醉中很常见.
- 确定PRAE可靠的生物标志物对于临床管理至关重要.
研究的目的:
- 研究miR-18a-5p作为儿童PRAE潜在生物标志物的作用.
- 探索miR-18a-5p在儿科麻醉中的诊断和预测价值.
主要方法:
- 分析了174名接受全身麻醉的儿童的临床数据.
- 在血清和细胞中使用qRT-PCR对miR-18a-5p表达的量化.
- 通过ROC曲线评估诊断价值,并使用后勤回归识别风险因素.
- 在体外实验中评估miR-18a-5p对肺细胞和炎症标志物的影响.
主要成果:
- 在患有PRAE的儿童中观察到升高的miR-18a-5p水平,显示出诊断相关性.
- miR-18a-5p表达与TNF-α和IL-6水平正相关.
- 年龄,肥胖,ASA等级和麻醉时间被确定为PRAE的独立风险因素.
- 静止miR-18a-5p改善了肺细胞活力,并减少了炎症反应.
结论:
- miR-18a-5p是小儿整体麻醉中PRAE的重要预测和诊断因素.
- 这些发现表明miR-18a-5p可能对管理PRAEs具有治疗潜力.
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