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设计一种可热激活的SpyCatcher/SpyTag蛋白质结合,用于可注射和在现场形成的水凝

Jian Cui¹, Yuan Gao¹, Chenjing Lu¹

¹Collaborative Innovation Center of Advanced Microstructures, National Laboratory of Solid State Microstructures, Department of Physics, Nanjing University, Nanjing, 210093, China.

Angewandte Chemie (International ed. in English)

|November 29, 2025

概括

研究人员开发了一种可热激活的SpyCatcher/SpyTag (TASpy) 系统,用于创建强大的可注射蛋白质水凝. 这个系统在体温下迅速形成水凝,提高机械强度和细胞活力,用于组织工程和药物输送.

科学领域:

生物材料科学生物材料科学
化学工程是化学工程的重要组成部分.
分子生物学分子生物学

背景情况:

可注射的水凝对于组织工程和药物输送至关重要.
目前的水凝在平衡凝速度,机械强度和细胞相容性方面面临着挑战.

研究的目的:

开发一种新型的可热激活的SpyCatcher/SpyTag (TASpy) 系统,用于快速的in situ水凝形成.
为了创建强大的,可注射的蛋白质水凝,具有改进的特性.

主要方法:

通过将SpyCatcher与非反应性的SpyTag突变体融合,设计了一个可热激活的SpyCatcher (TASpyC).
利用CnaB2域的灵感来实现温度依赖的展开和共价结合.
研究了水凝的特性,包括凝动力学,机械强度,细胞封装和体内性能.

主要成果:

在生理温度 (37°C) 上,TASpy系统证明了快速的化学交联.
实现了增强的机械性能,改善了细胞封装,并在加热后加速凝.
塔斯皮水凝表现出高细胞活力,在体内保持良好,以及生物相容性,皮下注射在几分钟内凝固.

结论:

塔斯皮系统为开发先进的注射蛋白质水凝提供了一个有前途的方法.
这项技术解决了生物医学应用当前水凝系统的关键局限性.
由于其快速形成,机械强度和生物相容性,TASpy水凝对组织工程和药物输送应用具有显著的潜力.

关键词:

细胞封装的封装方式化学交叉连接化学交叉连接可以注射的水凝.这就是SpyCatcher.热激活蛋白质结合热激活蛋白质结合

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