通过全细胞生物转化从PET单体乙烯基醇生产甘油酸,使用工程Corynebacterium glutamicum
Mohammad Rifqi Ghiffary1,2, Fong Tian Wong1,2,3, Yee Hwee Lim1,2,4
1Institute of Sustainability for Chemicals, Energy and Environment (ISCE2), Agency for Science, Technology and Research (A*STAR), 1 Pesek Road, Jurong Island, Singapore 627833, Republic of Singapore.
这项研究设计了Corynebacterium glutamicum,将乙烯基醇 (EG) 转化为有价值的甘油酸 (GA). 生物转化平台实现了高产量,为化学品生产和塑料废物利用提供了可持续的途径.
科学领域:
- 生物技术是生物技术.
- 合成生物学 合成生物学
- 化学工程是化学工程的重要组成部分.
背景情况:
- 全球变暖和塑料污染需要从替代原料中生产可持续的化学品.
- 乙烯基醇 (EG),一种聚乙烯基四甲酸盐 (PET) 单体,是微生物化学合成的潜在可再生基质.
研究的目的:
- 使用Corynebacterium glutamicum开发一个生物转化平台,从EG中生产甘油酸 (GA).
- 为了设计C. glutamicum以实现高效的EG转化并优化GA生产.
主要方法:
- 通过引入异构的EG氧化途径来改造C. glutamicum.
- 采用促进者工程来创建一个高产品种 (FA4).
- 实施了使用休息细胞进行增强生产的两阶段生物转化策略.
主要成果:
- 工程菌株FA4在48小时内从EG产生10.6g/L的GA.
- 通过两阶段的休息细胞策略,在72小时内达到98.8g/L的累计GA标位.
- 该平台成功地在72小时内从模拟的PET降解EG混合物中产生了67.3g/L的GA.
结论:
- 科里尼细菌 (Corynebacterium glutamicum) 作为一个高效的底盘,可以从EG中生产可持续的GA.
- 这种生物转化平台展示了将PET塑料废弃物价值化为有价值化学品的有前途途途.
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