简单的双不对称收缩扩张阵列集成芯片在线与ICP-MS相结合,用于循环瘤细胞的高通量计数
Xuelian Fu1, Beibei Chen1, Man He1
1Department of Chemistry, Wuhan University, Wuhan 430072, China.
Analytical chemistry
|November 29, 2025
概括
一个新的微流体芯片与ICP-MS结合,快速隔离并检测血液中的循环瘤细胞 (CTC). 这种方法在去除白细胞 (WBCs) 中显示出高效率,并准确地计数CTCs,与癌症阶段相对应.
科学领域:
- 生物医学工程 生物医学工程
- 分析化学 分析化学
- 在瘤学瘤学.
背景情况:
- 循环瘤细胞 (CTC) 是癌症早期检测的重要生物标志物.
- 诱导合等离子体质谱 (ICP-MS) 为细胞分析提供高灵敏度和特异性.
- 将微流体分类与ICP-MS集成为临床诊断中CTC检测提供了一个有希望的途径.
研究的目的:
- 开发一个集成的芯片-ICP-MS平台,以快速隔离和检测CTC.
- 通过有效地去除白细胞 (WBCs) 来提高CTC纯度.
- 建立与癌症分期相关的CTC计数量的定量方法.
主要方法:
- 一个双不对称的收缩-扩张阵列 (ACEA-ACEA) 微流体芯片被设计用于协同惯性提升和基于Dean力的细胞分类.
- 欧洲 (Eu) 标签被用于通过ICP-MS对CTC的基本检测.
- 综合系统在线进行血液样本中CTC的分离,丰富和检测.
主要成果:
- 该ACEA-ACEA芯片实现了高吞吐量 (300μL/分钟),CTC恢复 (94.18%) 和WBC去除效率 (99.46%).
- 癌症患者的CTC计数在10至176细胞/毫升之间,在健康个体中没有检测到CTC.
- 检测到的CTC数量与患者TNM分期呈正相关性.
结论:
- 开发的ACEA-ACEA芯片-ICP-MS平台可以实现高通量,灵敏和准确的CTC检测.
- 这种方法在推进癌症诊断和监测方面具有重大潜力.
- 与TNM分期的相关性强调了它对评估癌症进展的临床相关性.
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