基于亚基因组特异性重复的小麦D基因组特异性分子标记物的开发
Tatiana V Danilova1, Ahmed Charif2, Katherine Frels2
1USDA-ARS, Wheat, Sorghum & Forage Research Unit, Lincoln, NE, 68583, USA. tatiana.danilova@usda.gov.
概括
研究人员开发了新的分子标记物,以检测小麦中的D基因组染色体,帮助基因侵入作物改进. 这些基于重复的标记物有助于识别来自相关物种的有价值的遗传物质.
科学领域:
- 遗传学和分子生物学
- 植物育种和基因组学
- 生物信息学是一种生物信息学.
背景情况:
- 在Triticeae中广泛的杂交使小麦通过基因内进而得到改善.
- 重复序列构成了Triticeae基因组的85%以上,是检测内进染色素的关键.
研究的目的:
- 开发D基因组特异性的分子标记物,用于检测小麦中的内进染色素.
- 为了识别和表征特定物种的重复序列,用于标记器的发展.
主要方法:
- 使用 RepeatExplorer 管道对低覆盖度序列数据进行比较分析.
- 基于D基因组特异性SNP的PCR基因竞争扩展 (PACE) 试验的开发.
- 对内进群的查和使用光 in situ 杂交的确认.
主要成果:
- 确定了三种适用于D基因组标记物开发的重复类型 (Quinta,Afa家族,Tekay血统).
- 成功设计和应用PACE测试来检测小麦中的D基因组染色体.
- 在识别内进分段和染色体方面确认了标记器的准确性.
结论:
- 基于重复的分子标记器提供了一种有效的方法来检测D基因组染色素.
- 开发的方法有助于在小麦育种计划中识别内进染色素.
- 这种策略可以适应在不同染色体组中开发任何内进侵袭的标记物.
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