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DNA Isolation

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.

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相关实验视频

Updated: May 6, 2026

An Easy Method for Plant Polysome Profiling

An Easy Method for Plant Polysome Profiling

Published on: August 28, 2016

采用直角有机相分离方法从植物中捕获RNA结合蛋白质的协议.

Victor A Sánchez-Camargo¹, Gertjan Kramer², Harrold A van den Burg³

¹Molecular Plant Pathology, Swammerdam Institute for Life Sciences, University of Amsterdam, 1098 XH Amsterdam, the Netherlands.

|November 29, 2025

概括

这项研究引入了一种新协议,以分离与RNA相关联的RNA结合蛋白 (RBPs). 这种方法有助于通过植物蛋白质组学分析了解RBP功能.

关键词:

分子生物学分子生物学植物科学植物科学蛋白质的生物化学蛋白质的生物化学

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mRNA Interactome Capture from Plant Protoplasts

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A Stepwise Guide to the Isolation and Analysis of Leaf Surface and Apoplastic RNA Using Arabidopsis Rosettes

A Stepwise Guide to the Isolation and Analysis of Leaf Surface and Apoplastic RNA Using Arabidopsis Rosettes

Published on: August 8, 2025

相关实验视频

Last Updated: May 6, 2026

An Easy Method for Plant Polysome Profiling

An Easy Method for Plant Polysome Profiling

Published on: August 28, 2016

mRNA Interactome Capture from Plant Protoplasts

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

A Stepwise Guide to the Isolation and Analysis of Leaf Surface and Apoplastic RNA Using Arabidopsis Rosettes

A Stepwise Guide to the Isolation and Analysis of Leaf Surface and Apoplastic RNA Using Arabidopsis Rosettes

Published on: August 8, 2025

科学领域:

分子生物学分子生物学
蛋白质组学是指蛋白质组学.
植物科学植物科学

背景情况:

RNA结合蛋白 (RBPs) 是RNA处理的关键调节者,包括合成,功能,运输和衰变.
了解RBP与RNA的相互作用对于破译基因调节至关重要.
现有的分离RNA-蛋白质复合体的方法可能是有限的.

研究的目的:

提出一种用于将与RNA交联的RNA结合蛋白质 (RBPs) 大量分离的新方案.
为这些复合体的后续蛋白质组学分析制定战略.
提供一种适用于各种植物组织的方法.

主要方法:

在体内进行紫外线交联以稳定RNA-蛋白相互作用.
组织溶解和分离以准备样本.
使用有机溶剂净化交联RNA-蛋白质添加物.
蛋白质组学分析以确定结合的蛋白质.

主要成果:

成功开发了一种用于大量分离交联RNA-蛋白质复合物的综合性协议.
该协议使随后的蛋白质组学分析能够识别RBP.
在Nicotiana benthamiana叶子中已证明适用性,具有更广泛植物应用的潜力.

结论:

开发的协议提供了一种有效的方法来分离与RNA交联的RNA结合蛋白 (RBPs).
这种技术有助于在植物中进行RNA-蛋白相互作用的大规模蛋白质组学研究.
该协议可以适应各种植物物种和组织,推进RBP研究.