使用DNA条形码与单细胞RNA测序结合的DNA条形码追踪单细胞衍生克隆的协议

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Hee Jin Shin¹, Sahil Sharma², Sarah Kronheim²

¹Department of Medical Biophysics, University of Toronto, Toronto, ON M5G 1L7, Canada.

|November 29, 2025

概括

这项研究提出了一种新的协议,用于使用DNA条形码和单细胞RNA测序来跟踪细胞克隆. 这种方法将克隆生长与基因表达联系起来,有助于癌症研究.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

由克隆体适应性和可塑性驱动的瘤异质性,有助于癌症的进展和治疗耐药性.
了解克隆动态对于开发有效的癌症疗法至关重要.

研究的目的:

提供一个详细的协议,用于跟踪来自独特标记细胞的克隆输出.
将克隆生长活动与转录基因特征联系起来,以获得更深入的生物学见解.

主要方法:

构建和验证lentiviral DNA条形码库.
单细胞RNA测序 (scRNA-seq) 用于分析转录组形状.
永久的细胞标签用于长期的克隆追踪.

主要成果:

该协议使细胞能够永久标记具有独特DNA条形码的细胞.
它成功地将克隆生长和扩张与特定的转录基因特征联系起来.
这种方法有助于研究各种模型系统中的克隆动态.

结论:

该协议为研究癌症中克隆功能的分子机制提供了一个强大的工具.
它提供了关于瘤异质性,疾病进展和治疗耐药性的见解.
该方法通过阐明克隆行为,支持开发向癌症疗法.

关键词:

癌症癌症癌症癌症细胞生物学细胞生物学在RNA-seqqq.序列分析是指进行序列分析.序列化是指测序的使用.一个单细胞的单细胞.

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