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相关概念视频

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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相关实验视频

Updated: Jan 10, 2026

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
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使用DNA条形码与单细胞RNA测序结合的DNA条形码追踪单细胞衍生克隆的协议.

Hee Jin Shin1, Sahil Sharma2, Sarah Kronheim2

  • 1Department of Medical Biophysics, University of Toronto, Toronto, ON M5G 1L7, Canada.

STAR protocols
|November 29, 2025
PubMed
概括

这项研究提出了一种新的协议,用于使用DNA条形码和单细胞RNA测序来跟踪细胞克隆. 这种方法将克隆生长与基因表达联系起来,有助于癌症研究.

关键词:
癌症 癌症 癌症 癌症细胞生物学 细胞生物学在RNA-seqqq.序列分析是指进行序列分析.序列化是指测序的使用.一个单细胞的单细胞.

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相关实验视频

Last Updated: Jan 10, 2026

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科学领域:

  • 在瘤学瘤学.
  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.

背景情况:

  • 由克隆体适应性和可塑性驱动的瘤异质性,有助于癌症的进展和治疗耐药性.
  • 了解克隆动态对于开发有效的癌症疗法至关重要.

研究的目的:

  • 提供一个详细的协议,用于跟踪来自独特标记细胞的克隆输出.
  • 将克隆生长活动与转录基因特征联系起来,以获得更深入的生物学见解.

主要方法:

  • 构建和验证lentiviral DNA条形码库.
  • 单细胞RNA测序 (scRNA-seq) 用于分析转录组形状.
  • 永久的细胞标签用于长期的克隆追踪.

主要成果:

  • 该协议使细胞能够永久标记具有独特DNA条形码的细胞.
  • 它成功地将克隆生长和扩张与特定的转录基因特征联系起来.
  • 这种方法有助于研究各种模型系统中的克隆动态.

结论:

  • 该协议为研究癌症中克隆功能的分子机制提供了一个强大的工具.
  • 它提供了关于瘤异质性,疾病进展和治疗耐药性的见解.
  • 该方法通过阐明克隆行为,支持开发向癌症疗法.