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相关概念视频

CRISPR01:59

CRISPR

57.4K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
18.7K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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相关实验视频

Updated: Jan 9, 2026

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
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合成型III-ECRISPR-Cas效应器用于可编程RNA准.

Daniel J Brogan1, Calvin P Lin2, Elena Dalla Benetta1

  • 1School of Biological Sciences, Department of Cell and Developmental Biology, University of California San Diego, La Jolla, CA 92093, USA.

Journal of molecular biology
|November 29, 2025
PubMed
概括

研究人员通过在III-E型系统内交换域来设计了一种新的CRISPR-Cas效应器. 这一发现为创建基于自然设计的RNA向卡斯效应器提供了新的方法.

关键词:
这就是CRISPR-Cas.通过RNA的淘汰,RNA的淘汰.蛋白质工程工程 蛋白质工程类型III-E的效应器

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Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx
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Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx

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In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
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In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

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相关实验视频

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CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
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Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx
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In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
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科学领域:

  • 分子生物学分子生物学
  • 遗传学 遗传学 是一个
  • 生物化学 生物化学

背景情况:

  • 克里斯普尔-卡斯系统对于 prokaryotes 的适应性免疫是至关重要的.
  • 类型III-E CRISPR-Cas效应器类,最近发现的一组,由合的Cas7和Cas11域组成,用于RNA向.
  • 了解这些系统的模块化和进化灵活性是利用其潜力的关键.

研究的目的:

  • 为了识别和描述新型III-E类CRISPR-Cas效应器.
  • 调查III-E类效应器的域模块化和工程潜力.
  • 开发一种新的方法来创建嵌合式RNA向的Cas效应器.

主要方法:

  • 对新型CRISPR-Cas效应子序列的生物信息识别.
  • 蛋白质域分析和类型III-E效应器组件的比较.
  • 通过域互换和添加工程化仿真CRISPR-Cas效应器的功能特征.

主要成果:

  • 确定了一种新型III-E类效应器,包括三个Cas7域和一个Cas1域.
  • 这种新型效应器是不活跃的,但成功地被改造成一个活跃的RNA向的Cas效应器.
  • 域互换实验表明,在III-E型效应器架构中,具有显著的灵活性,包括成功交换Cas1和Cas11域.

结论:

  • 类型III-E CRISPR-Cas效应器域表现出显著的模块化,允许不同效应器之间的功能互换.
  • 类型III-E效应器的自然蓝图为工程新型,活跃的RNA向Cas系统提供了一个框架.
  • 这项工作通过利用域可塑性来设计和设计CRISPR-Cas工具的新范式.