外源的CaCl2增强了Cucumis sativus L.在通过蛋白质S-Nitrosylation通过的压力下进行的偶然根形成
Lijuan Niu1, Yanshuo Zhao1, Qian Wang1
1College of Life Sciences and Agri-forestry, Southwest University of Science and Technology, Mianyang, Sichuan, 621010, China.
Plant science : an international journal of experimental plant biology
|November 29, 2025
概括
离子 (Ca2+) 通过依赖氧化 (NO) 的S-化,在 (Cd) 压力下促进黄瓜的偶然根植. 这个过程涉及关键蛋白质的S-化,增强植物的应激耐受性和根部发育.
科学领域:
- 植物生物学 植物生物学
- 环境压力生理学生理学
- 分子植物科学 分子植物科学
背景情况:
- (Cd) 压力抑制了植物的生长,影响了根部的发育.
- 离子 (Ca2+) 和氧化 (NO) 是植物应激反应中至关重要的信号分子.
- 在Cd压力下,Ca2+,NO和偶然根植之间的相互作用尚未得到充分理解.
研究的目的:
- 调查Ca2+在Cd压力下的黄瓜中促进偶然根植的作用.
- 阐明氧化物 (NO) 和S-化在Cd压力下的Ca2+介导的根部发育中的参与.
- 在Cd压力下对Ca2+治疗反应中受到S-化作用的关键蛋白质的识别.
主要方法:
- 在Cd压力下对黄瓜植物进行剂量依赖的CaCl2的应用.
- 使用Ca2+化剂和NO吸收剂来评估信号通路.
- 测量内源NO和总S-尼托索醇 (SNO) 的水平.
- 蛋白质组分析以识别S-化蛋白质.
- 酶活性测定用于评估去化剂的影响.
主要成果:
- 在剂量取决的方式上,Ca2+显著增强了偶然的根部发育,在200μM的CaCl2下获得最佳结果.
- Ca2+诱导的根促进依赖于内源的NO产量,并涉及增加的S-尼特罗斯醇 (SNO) 水平.
- 蛋白质组分析确定了117种S-化蛋白质,主要涉及氧化减氧,代谢和糖溶性过程.
- 脱化剂降低了关键酶的活性,这些酶对于在压力下偶然植根至关重要.
结论:
- 在Cd压力下,Ca2+在促进黄瓜的偶然根植中发挥着不可或缺的作用.
- 由Ca2+介导的偶然根植的促进取决于氧化 (NO) 信号通路.
- 关键蛋白质的S-化是一种关键的分子机制,是Ca2+诱导的偶然根形成和植物适应Cd压力的基础.
更多相关视频
相关概念视频
C4 Pathway and CAM
48.6K
Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
C4 Pathway
The C4 pathway is used by plants such as...
48.6K
Adaptations that Reduce Water Loss
27.8K
Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.
27.8K
Key Elements for Plant Nutrition
23.9K
Like all living organisms, plants require organic and inorganic nutrients to survive, reproduce, grow and maintain homeostasis. To identify nutrients that are essential for plant functioning, researchers have leveraged a technique called hydroponics. In hydroponic culture systems, plants are grown—without soil—in water-based solutions containing nutrients. At least 17 nutrients have been identified as essential elements required by plants. Plants acquire these elements from the...
23.9K


