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Wenqiong Zhang¹, Wenkui Mi², Jiahao Hu¹

¹Department of Environmental Science and Engineering, Guangdong Technion-Israel Institute of Technology, Shantou 515063, China; Faculty of Civil and Environmental Engineering, Technion, Haifa 32000, Israel.

Bioresource technology

|November 29, 2025

概括

可以减少使用混合微生物培养 (MMC) 进行多基酸盐 (PHA) 生产的能源密集型通风. 解测序批量反应器 (D-SBR) 中的无氧条件显著改善了PHA产量,并有利于特定的微生物群落进行高效的生物聚合物合成.

科学领域:

生物技术是生物技术.
环境微生物学环境微生物学
聚合物科学聚合物科学

背景情况:

使用交替有氧/无氧混合微生物培养 (MMC) 进行传统的聚氨酸酸盐 (PHA) 生产,需要大量的能量用于通风,从而阻碍了工业的可扩展性.
开发节能生物聚合物生产方法对于可持续的工业应用至关重要.

研究的目的:

在无氧条件下研究PHA生产的有效性,使用两种不同养策略的测序批量反应器 (SBR).
通过优化微生物培养操作来开发PHA合成的节能方法.

主要方法:

两个测序批量反应堆 (SBR),一个解 (D-SBR) 和一个合 (C-SBR) 的碳和营养料系统,在无氧条件下运行.
测量了多基酸盐 (PHA) 含量,并使用16S rRNA基因安普利康序列分析了微生物群体组成.

主要成果:

与C-SBR (10.9±3.4%) 相比,D-SBR实现了显著更高的细胞PHA含量 (50.6%在27天内,强大的产量为45.7±8.1%).
这两种系统都产生了富含3基酸盐的PHA (32.6%在D-SBR中,45.8%在C-SBR中).
微生物分析显示,与C-SBR (例如,Pandoraea,Thauera) 相比,D-SBR (例如,Defluviicoccus,Thauera,Brachymonas) 中存在不同的PHA生产社区.

结论:

基于无氧混合微生物培养的PHA生产是可行的,并提供潜在的成本降低.
分离的养策略和SBR中的营养限制可以提高PHA生产效率.
无氧条件有利于 propionate-to-3-hydroxyvalerate 生物合成,比有氧过程具有代谢优势.

关键词:

无毒PHA的生产生产.分离的碳和营养料.混合的微生物培养.聚酸酸多基甲酸

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