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类似P1的菌体驱动bla_CTX-M-27通过lysogenic转换和转换转换传输

Zengfeng Zhang¹, Chunlei Shi¹

¹MOST-USDA Joint Research Center for Food Safety, School of Agriculture and Biology, and State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, China.

International journal of antimicrobial agents

|November 29, 2025

概括

携带blaCTX-M-27基因的P1-类菌体通过宿主内部的转移转移抗微生物耐药性. 这些元素的溶解转化有助于物种间的传播,揭示了它们在ARG传播中的作用.

科学领域:

微生物学微生物学
遗传学是一个遗传学.
分子生物学分子生物学

背景情况:

类似P1的菌体等离子体 (PPs) 是混合的移动遗传元素,结合了菌体和等离子体的特性.
了解PPs介导的抗菌素耐药性基因 (ARG) 转移机制对于公共卫生至关重要.

研究的目的:

为了阐明ARG blaCTX-M-27中介于沙门氏菌印第安纳州P1-like PPs的转移机制.
调查P1类PP在ARG传播中的作用.

主要方法:

对S.Indiana分离物的抗微生物敏感性测试和基因组分析.
结合,性感染,溶解转化和传输电子显微镜测试.
转换试验和基因组步行分析Tn1722衍生物及其插入位.

主要成果:

在9个携带P1元素的南印第安纳州分离物中,有6个携带了与P1-like PPs相关的blaCTX-M-27基因.
与P1相似的PP p14076B缺乏结合能力,携带了Tn1722衍生物与blaCTX-M-27并通过溶解转化促进了感染.
转换试验证实了Tn1722介导的blaCTX-M-27转移,受tnpA基因和插入部位特征的影响.

结论:

Tn1722衍生物通过转移调解blaCTX-M-27的宿主内转移.
通过类似P1的PP进行素转化,有助于ARG在物种间传播.
类似P1的PP在ARG的传播中起着重要作用,需要进一步研究.

关键词:

溶解性转化是一种溶解性转化.类似P1的菌体等离子体 Tn1722 衍生品的衍生品转载转载转载转载 bla ((CTX-M-27) 的时间.

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